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安徽长江水系黄鳝的群体遗传结构分析
引用本文:胡玉婷,江 河,胡 王,潘庭双,段国庆,凌 俊.安徽长江水系黄鳝的群体遗传结构分析[J].广东农业科学,2015,42(1):113-118.
作者姓名:胡玉婷  江 河  胡 王  潘庭双  段国庆  凌 俊
作者单位:安徽省农科院水产研究所,安徽合肥,230031
基金项目:国家公益性行业(农业)科研专项,安徽省农科院院长青年创新基金,安徽省农科院黄鳝创新团队,安徽省科技攻关项目
摘    要:为研究安徽长江水系黄鳝(Monopterus albus)的遗传多样性和群体遗传结构,测定了其6个地理群体(当涂、无为、繁昌、贵池、怀宁和望江)共178尾个体的线粒体DNA控制区部分序列.对其长度为556~558 bp的控制区同源序列进行分析,共检测到变异位点41个(变异率7.35%),单倍型56种.平均单倍型多样性和核苷酸多样性分别为0.694~0.954、0.00237~0.01382.群体分化指数(Fst)和基因流(Nm)分别为0.01974~0.87529、0.07124~24.82928,分子变异分析(AMOVA)中,群体间遗传变异占61.72%,表明黄鳝群体间具有明显的遗传分化.基于单倍型或群体间遗传距离的分子系统进化树均显示,6个地理群体分为两支:当涂与繁昌群体聚为一支,其余4群体聚为另一支.

关 键 词:黄鳝  控制区  长江  群体遗传结构
收稿时间:2014/7/23 0:00:00

Population genetic structure analysis of Monopterus albus from Anhui reaches in the Yangtze River
HU Yu-ting,JIANG He,HU Wang,PAN Ting-shuang,DUAN Guo-qing,LING Jun.Population genetic structure analysis of Monopterus albus from Anhui reaches in the Yangtze River[J].Guangdong Agricultural Sciences,2015,42(1):113-118.
Authors:HU Yu-ting  JIANG He  HU Wang  PAN Ting-shuang  DUAN Guo-qing  LING Jun
Abstract:To assess the genetic diversity and population structure of Monopterus albus, 178 individuals of 6 populations (DT, WW, FC, GC, HN, WJ) from Anhui reaches in the Yangtze River were investigated based on 556-558 bp nucleotide sequences of mtDNA partial control region. The sequences analysis results showed that 41 variable sites (mutation rates of 7.35%), 56 haplotypes were detected. The haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.694-0.954 and 0.00237-0.01382, respectively. Analysis of molecular variance (AMOVA) revealed that genetic variation among the populations was 61.72%. These results indicated that there were significant genetic differentiations among populations. NJ-tree based on haplotypes or genetic distances among populations showed that DT and FC populations were clustered as one, and other 4 population was as another independent cluster.
Keywords:Monopterus albus  control region  the Yangtze River  population genetic structure
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