| 东北地区不同宿主NDV的分离鉴定与系统发育进化分析 |
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| 引用本文： | 刘文斌,崔尚金,李秀云,刘立奎,张洪英.东北地区不同宿主NDV的分离鉴定与系统发育进化分析[J].畜禽业,2006(2):26-30. |
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| 作者姓名： | 刘文斌 崔尚金 李秀云 刘立奎 张洪英 |
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| 作者单位： | 1. 中国农科院哈尔滨兽医研究所,疫病诊断与流行病学中心,黑龙江,哈尔滨,150001
2. 长春市畜禽防疫工作总站 |
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| 摘 要： | 针对我国东北地区获得高免新城疫(NewcastleDisease,ND)抗体鸡群仍然发生新城疫(ND)的情况,应用分子流行病学技术,对所分离的18株鸡源新城疫病毒(NewcastleDiseaseVirus,NDV)和1株鸽源NDV进行系统发育进化分析。将NDV分离株通过差速离心纯化,以SDS-蛋白酶K(或Trizol法)提取病毒RNA,RT-PCR扩增其融合蛋白(F)基因532bp或280bp关键片断,经回收克隆到pMD18-T载体上进行核苷酸序列测定(GenBank序列号:AY208680-AY208698)。核苷酸和氨基酸序列进行同源性比较,结果表明各毒株之间核苷酸同源性为83.1%～100%,氨基酸同源性为85.5%～100%;系统发育进化树分析显示,其中JL-12、HLJ-3为弱毒株,与疫苗株V4同属于基因Ⅰ型;其余17株均为强毒株,其中HLJ-4、JL-14为Ⅵ型,其余15株均为基因Ⅶ型,由此可见东北地区目前新城疫的流行是由3种不同基因型的NDV毒珠(Ⅰ型、Ⅵ型、Ⅶ型)所发,但以基因Ⅶ型为主,这与我国其他地区和世界上其他国家ND的流行情况趋于一致。
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| 关 键 词： | NDV F基因 分子流行病学 系统发育进化树 |
| 文章编号： | 1008-0414(2006)01(下)-0026-05 |
| 修稿时间： | 2005年10月9日 |
Epidemiological and phylogentic analysis of NDV isolated in northeast china |
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| LIU Wen-bin,CUI Shang-jin,LI Xiu-yun,LIU Li-kui,ZHANG Hong-ying.Epidemiological and phylogentic analysis of NDV isolated in northeast china[J].Livestock and Poultry Industry,2006(2):26-30. |
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| Authors: | LIU Wen-bin CUI Shang-jin LI Xiu-yun LIU Li-kui ZHANG Hong-ying |
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| Abstract: | In recent years,there occurred non-typical ND in vaccinated chicken flock of northeast china.To have insight into the molecular epidemiology of Newcastle disease virus(NDV)prevailed.18 NDV strains isolated from chicken and 1 NDV isolated from pigeon using SPF chicken embryos were studied with RT-PCR and subsequence analysis of the region of 255 nucleotides covering the fusion(F)protein cleavage site(GenBank number:AY208680-AY208698).JL-12,HLJ-3 have the amino acid sequence 112GR(K)QGRL117 at the F2/F1 cleavage site(112-117)of F protein,which confirmed that they were lentogenic,the other isolated NDV strains have 112RRQR(K)RF117,which confirmed that they were velogenic.The genetic similarities among the viruses isolated are 83.1%～100%,protein similarities among the viruses are 85.5%～100%.Phylogenetic tree based on 255 bp(position 338～592 nt)region of F gene was established.The tree indicated JL-12,HLJ-3 were of genotypeⅠthe same as V4;HLJ-4,JL-14 were of genotype Ⅵ.Other 15 strains were grouped under genotype Ⅶ.All the results indicated that NDV in northeast china is very complex,Both old genotypeⅠ(2/19)and Ⅵ(2/19)and novel Ⅶ(15/19)had been isolated and genotype Ⅶ were most prevailing strains. |
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| Keywords: | NDV |
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