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| 捕食线虫性真菌18S rDNA基因序列与种系发生关系研究 | |
| 引用本文： | 杨莲茹,杨晓野,刘珍莲,汪明,考桂兰,刘海成,刘军.捕食线虫性真菌18S rDNA基因序列与种系发生关系研究[J].畜牧兽医学报,2004,35(3):329-333. |
| 作者姓名： | 杨莲茹 杨晓野 刘珍莲 汪明 考桂兰 刘海成 刘军 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018 2. 中国农业大学动物医学院,北京,100094 3. 巴彦淖尔盟兽医工作站,临河,015000 4. 内蒙古包头市畜牧兽医工作站,包头,014010 |
| 基金项目： | 国家自然科学基金资助项目(30260081) |
| 摘 要： | 对捕食线虫性真菌——少孢节丛孢菌A1分离株(Arthrobotrys oligospora A1)的18S rDNA基因序列进行了研究。结果表明：其18S rDNA全基因序列为1769bp，在进化树上与同种国外分离株A．oligospora var oligospora 1最为接近，同源性为94．7％。这与二者都具有捕食性结构—菌网的特点相一致。证实捕食线虫性真菌的捕食性结构与捕食线虫性真菌种系发生有关。从少孢节丛孢菌3个分离菌株(Arthrobotrys oligospora A1、A．oligospora 1、A．oligospora2)的进化树及同源性来看。不同国家地区的丛孢菌属(Arthrobotrys)分离株确实存在差异。 |
| 关 键 词： | 捕食线虫性真菌 18S rDNA基因 序列分析 种系发生 少孢节丛孢菌 捕食性结构 分类 |
| 文章编号： | 0366-6964(2004)-03-0329-05 |
Study of the Relation on the 18S Rdna Gene Sequence and the Phylogeny of Nematode-trapping Fungi |
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| YANG Lian-ru,YANG Xiao-ye,LIU Zhen-lian,WANG Ming,KAO Gui-lan,LIU Hai-cheng,LIU Jun.Study of the Relation on the 18S Rdna Gene Sequence and the Phylogeny of Nematode-trapping Fungi[J].Acta Veterinaria et Zootechnica Sinica,2004,35(3):329-333. | |
| Authors: | YANG Lian-ru YANG Xiao-ye LIU Zhen-lian WANG Ming KAO Gui-lan LIU Hai-cheng LIU Jun |
| Institution: | YANG Lian-ru~1,YANG Xiao-ye~1,LIU Zhen-lian~1,WANG Ming~2,KAO Gui-lan~1,LIU Hai-cheng~3,LIU Jun~4 |
| Abstract: | The 18S rDNA gene sequence and the phylogeny of nematode-trapping fungus such as the isolate of Arthrobotrys oligospora A1 were studied. The results showed that the full 18S rDNA gene of Arthrobotrys oligospora A1 contained 1769bp nucleotides. The phylogenic analysis of 18S rDNA gene sequence from nematode-trapping fungus reported in GenBank and our isolate was conducted by DNASTAR and evolution trees were established. In evolution trees Arthrobotrys oligospora A1 showed a relatively closer relationship with A.oligospora var. oligospora 1, the homology of the nucletide sequence of them was 94.7%, which was in agreement with their trappiny organs-net works. It indicated that the trapping devices might be related with the phylogenic evolution of nematode-trapping fungi. It implied that they may belong to different strains from the areas of different countries based on the evolution tree and the homogeny result of 3 isolates of Arthrobotrys oligospora (Arthrobotrys oligospora A1, A.oligospora 1, A.oligospora 2)in this study. |
| Keywords: | Nematode-trapping fungi Arthrobotrys oligospora Nematode-trapping organ 18S rDNA gene Phylogeny |
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