| 大蒜未受精子房离体诱导单倍体的研究 |
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| 引用本文： | 刘颖颖,刘世琦,薛小艳,刘景凯,陈祥伟,冯磊.大蒜未受精子房离体诱导单倍体的研究[J].园艺学报,2013,40(6):1178. |
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| 作者姓名： | 刘颖颖 刘世琦 薛小艳 刘景凯 陈祥伟 冯磊 |
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| 作者单位： | （山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，农业部黄淮地区园艺作物生物学与种质创制重点实验室，山东泰安 271018） |
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| 基金项目： | 国家公益性行业科研专项，山东省农业重大创新项目 |
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| 摘 要： | 以苍山大蒜品种‘糙蒜’为试材，通过花茎离体培养获得发育健壮的花器官。以其未受精子房为外植体进行离体培养，研究不同培养基（MS、B5、N6）及外源植物生长调节剂对单倍体诱导的影响，以建立大蒜单倍体诱导体系。结果表明，诱导未受精子房产生愈伤组织的适宜培养基为B5 + 2 mg · L-1 6-BA + 1 mg · L-1 NAA，愈伤组织及雌核发育胚总诱导率可达12.24%；诱导愈伤组织分化不定芽的适宜培养基为B5 + 3 mg · L-1 6-BA + 1 mg · L-1 NAA，不定芽分化频率可达46.15%；将不定芽转移至B5 + 0.05 mg · L-1 NAA培养基中可诱导生根，发育成完整植株。以再生植株叶片为材料，采用流式细胞仪进行倍性鉴定，所得18株再生植株中有16株为单倍体植株。
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| 关 键 词： | 大蒜 单倍体 未受精子房 流式细胞仪 |
| 收稿时间： | 2013-02-05 |
Studies on Haploid Plant Induction via in Vitro of Unfertilized Ovary of Garlic |
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| LIU Ying-ying,LIU Shi-qi,XUE Xiao-yan,LIU Jing-kai,CHEN Xiang-wei,FENG Lei.Studies on Haploid Plant Induction via in Vitro of Unfertilized Ovary of Garlic[J].Acta Horticulturae Sinica,2013,40(6):1178. |
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| Authors: | LIU Ying-ying LIU Shi-qi XUE Xiao-yan LIU Jing-kai CHEN Xiang-wei FENG Lei |
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| Institution: | （College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Agriculture Ministry Key Laboratory of Biology and Genetic Improvement of Horticultural Crop(Huanghuai Region)，Tai’an，Shandong 271018，China） |
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| Abstract: | The flower buds of garlic（Allium sativum L.） were used to get well-developed floral organ by means of garlic stem culture in vitro. With the unfertilized ovary，a regeneration system of garlic in vitro was established by analysis the effects of different medium（MS，B5，N6）and different hormones on haploid induction. The suitable media for callus induction was B5 + 2 mg · L-1 6-BA + 1 mg · L-1 NAA，the highest induction rate of callus and gynogenic embryos was 12.24%；The suitable media for adventitious bud was B5 + 3 mg · L-1 6-BA + 1 mg · L-1 NAA，the regeneration frequencies was 46.15%；Adventitious bud and embryos could grow root and develop into complete plant in the media B5 + 0.05 mg · L-1 NAA. Eighteen regeneration plants were obtained in total. Ploidy analysis of the regenerations with flow cytometry analysis of leaf tissue revealed that 16 regeneration palnts are haploid. |
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| Keywords: | garlic haploid unfertilized ovary flow cytometry |
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