Protective efficacy of a live attenuated Mycoplasma hyopneumoniae vaccine with an ISCOM-matrix adjuvant in pigs |
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| Authors: | Qiyan Xiong Yanna Wei Zhixin Feng Yuan Gan Zhanjun Liu Maojun Liu Fangfang Bai Guoqing Shao |
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| Institution: | 1. School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, 464 Bearsden Road, Glasgow G61 1QH, UK;2. School of Life Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, 346 West Medical Building, Glasgow G12 8QQ, UK;3. Physical Activity for Health Group, School of Psychological Sciences and Health, Faculty of Humanities and Social Sciences, University of Strathclyde, Graham Hills Building, 40 George Street, Glasgow G1 1QE, UK |
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| Abstract: | An attenuated Mycoplasma hyopneumoniae vaccine that requires intrathoracic administration is commercially available for use against mycoplasmal pneumonia in China. Given the limitations of such a route of administration, this study was undertaken to assess the capacity of an ISCOM-matrix adjuvant to enhance immunogenicity following intramuscular use. Immune responses in pigs following vaccination and subsequent intra-tracheal bacterial inoculation were examined using lymphocyte proliferation, serology and mucosal IgA in both nasal and saliva swabs.Vaccination induced clear lymphocyte proliferation, but only slight serum antibody responses although these were significantly increased following experimental infection. Mucosal IgA was not detected in either nasal or salivary secretions. Following bacterial challenge, animals vaccinated with the adjuvant-containing live vaccine exhibited less severe pulmonary lesions (median score 3.67) than unvaccinated pigs (median score 13.58). The degree of ciliary loss on the respiratory tract surface was reduced in vaccinated pigs compared with experimentally infected controls. The findings indicated that the adjuvant vaccine administered IM provided protection against experimentally induced mycoplasmal pneumonia and could have commercial potential. |
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| Keywords: | Pig Adjuvant Intramuscular vaccine |
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