| 过氧化氢和甲醛对SPF级大鼠和小鼠繁殖室消毒效果观察 |
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| 引用本文： | 过氧化氢和甲醛对SPF级大鼠和小鼠繁殖室消毒效果观察.过氧化氢和甲醛对SPF级大鼠和小鼠繁殖室消毒效果观察[J].畜牧与饲料科学,2019,40(11):21-23. |
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| 作者姓名： | 过氧化氢和甲醛对SPF级大鼠和小鼠繁殖室消毒效果观察 |
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| 作者单位： | 宁夏医科大学实验动物中心,宁夏 银川 750004 |
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| 基金项目： | 宁夏医科大学校级项目（XY2017019） |
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| 摘 要： | 目的比较观察过氧化氢和甲醛对SPF级大鼠和小鼠繁殖室内表面、台面以及空气的消毒效果,选择消毒效果良好的消毒剂。方法采用空气落下菌检测法和表面细菌拭子检测法,对过氧化氢和甲醛熏蒸SPF级大鼠和小鼠繁殖室的消毒效果进行检测观察。结果SPF级大鼠和小鼠繁殖室分别经过氧化氢和甲醛熏蒸消毒后,进行空气落下菌的检测,过氧化氢和甲醛对大鼠繁殖室和小鼠繁殖室空气的杀菌率分别为100%、97.5%和100%、100%;将墙壁、地面和笼架表面的菌落数进行检测,过氧化氢和甲醛对大鼠繁殖室墙壁、地面和笼架的杀菌率分别为100%、99%、100%和95%、100%、99%,对小鼠繁殖室墙壁、地面和笼架的杀菌率均为100%。结论过氧化氢蒸汽消毒是比较理想的消毒方法,很好地控制了SPF级大鼠和小鼠繁殖室环境微生物,安全性高且消毒效果明显。
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| 关 键 词： | 过氧化氢 甲醛 SPF级大鼠和小鼠 繁殖室 消毒 |
| 收稿时间： | 2019-09-07 |
Disinfection Effects of Hydrogen Peroxide and Formaldehyde in Breeding Room ofBarrier SPF Rats and Mice |
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| WANG Xiao-dong,YANG Wen,CHEN Jian-mao,YANG Meng-meng,WANG Zhi-sheng.Disinfection Effects of Hydrogen Peroxide and Formaldehyde in Breeding Room ofBarrier SPF Rats and Mice[J].Animal Husbandry and Feed Science,2019,40(11):21-23. |
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| Authors: | WANG Xiao-dong YANG Wen CHEN Jian-mao YANG Meng-meng WANG Zhi-sheng |
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| Institution: | Laboratory Animal Center,Ningxia Medical University,Yinchuan 750004,China |
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| Abstract: | Objective] To compare the disinfection effects of hydrogen peroxide and formaldehyde on the interior surface, mesa and air of the breeding room of barrier SPF rats and mice, and to select effective disinfectants. Method] The disinfection effects of hydrogen peroxide and formaldehyde by fumigation on reproductive chambers of SPF rats and mice were observed via air falling bacteria detection method and swab method. Result] After fumigation with hydrogen peroxide and formaldehyde, the bacteria in the air of SPF rats breeding room and mice breeding room were detected. The sterilization rates of hydrogen peroxide and formaldehyde in the air of rats breeding room and mice breeding room were 100%, 97.5% and 100%, 100%, respectively. The bacterial colonies on the walls, floors and cage surfaces were detected for peroxidation. The bactericidal rates of hydrogen peroxide and formaldehyde on the walls, floors and cage surfaces of rats reproductive chambers were 100%, 99%, 100% and 95%, 100%, 99%, respectively, and those of mice reproductive chambers were all 100%. Conclusion] Steam disinfection with hydrogen peroxide is an ideal disinfection method, which can effectively control the microorganisms in the breeding room of SPF rats and mice and has high safety and obvious disinfection effect. |
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| Keywords: | hydrogen peroxide formaldehyde SPF rats and mice breeding room disinfection |
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