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2014-2019年青藏高原牦牛轮状病毒血清学检测与分析
引用本文:周赛赛,宋仁德,罗晓林,赵索南,马进寿,参木友,石红梅,索朗斯珠.2014-2019年青藏高原牦牛轮状病毒血清学检测与分析[J].中国动物传染病学报,2021(1).
作者姓名:周赛赛  宋仁德  罗晓林  赵索南  马进寿  参木友  石红梅  索朗斯珠
作者单位:西藏农牧学院动物科学学院;青海玉树藏族自治州畜牧兽医工作站;四川省草原科学研究院;青海省海北畜牧兽医科学研究所;青海省大通种牛场;西藏农牧科学院草原研究所;甘南州畜牧工作站
基金项目:国家肉牛牦牛产业体系项目(CARS-37)。
摘    要:为明确青藏高原4个省区牦牛轮状病毒(RV)的流行情况,本研究采用双抗原一步夹心ELISA法,对2014-2019年采集自西藏、青海、甘肃、四川的630份牦牛血清样品进行了RV抗体检测。结果显示:630份青藏高原牦牛RV血清抗体总阳性率为18.57%(117/630),西藏地区牦牛RV抗体阳性率与其他地区相比较高;2014-2019年青藏高原地区牦牛RV血清抗体年阳性率分别为37.78%、7.62%、17.14%、14.17%、15.00%、13.33%,平均阳性率为18.57%;4省牦牛RV抗体阳性率分别为西藏(25.76%)、青海(8.33%)、甘肃(16.67%)、四川(11.67%);4月龄以下牦牛(32.27%)极显著高于4~12月龄(14.05%)和大于12月龄(9.76%)。本研究结果为了解青藏高原地区牦牛轮状病毒病的血清流行病学情况奠定了坚实基础。

关 键 词:青藏高原  牦牛  轮状病毒  血清学  检测学分析

Serological Detection and Analysis of Yak Rotavirus in Qinghai-Tibet Plateau from 2014 to 2019
Institution:(Animal Science College,Tibet Agriculture and Animal Husbandry University,Linzhi 860000,China;Livestock and Veterinary Workstation,Yushu Tibetan Autonomous Prefecture,Yushu 815400,China;Sichuan Grassland Science Research Institute,Chengdu 610097,China;Institute of Animal Husbandry and Veterinary Sciences of Haibei Qinghai Province,Haibei 812200,China;Datong Cattle Farm,Datong 810102,China;Grassland Research Institute,Tibetan Academy of Agricultural Sciences,Lasa 850000,China;Gannan Animal Husbandry Workstation,Gannan 747000,China)
Abstract:To investigate the prevalence of Rotavirus(RV)infection in yaks in 4 provinces of Qinghai-Tibet plateau.Total 630 yak serum samples were collected during 2014-2019 and tested using double antigen sandwich ELISA.The results showed that the annual positive rates of RV serum antibodies in the Qinghai-Tibet plateau were 37.78%,7.62%,17.14%,14.17%,15.00%,and 13.33%in these years with the average positive rate at 25.76%.The yak RV antibody positive rates were 25.76%in Tibet,8.33%in Qinghai,16.67%in Gansu and 11.67%in Sichuan.In addition,the positive rate under 4 months old yaks was significantly higher than those between 4-12 months and over 12 months old.In conclusion,total positive rate of serum antibodies in yaks were 18.57%(117/630)and higher in Tibet than other regions.This study provided seroepidemiological data of yak rotavirus disease in Qinghai-Tibet plateau.
Keywords:Qinghai-Tibet  yak  Rotavirus  epidemiology  detection and analysis
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