| 猪繁殖与呼吸综合征病毒BD株的分离及GP4基因的克隆 |
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| 引用本文： | 王金凤,张会民,袁万哲,孙继国.猪繁殖与呼吸综合征病毒BD株的分离及GP4基因的克隆[J].广东畜牧兽医科技,2011,36(3):33-35. |
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| 作者姓名： | 王金凤 张会民 袁万哲 孙继国 |
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| 作者单位： | 河北农业大学动物科技学院,河北保定,071001 |
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| 摘 要： | 从河北保定分离到一株疑似猪繁殖与呼吸综合征病毒,接种Marc-145细胞,经过两代盲传后,出现细胞病变,经鉴定为PRRSV,命名为BD株.根据GenBank公布的PRRSV JXA1株0RF4基因的核苷酸序列,设计并合成一对特异性引物,用RT-PCR方法扩增完整ORF4基因,将扩增产物连接到pMD19-T 载体并转化克...
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 BD株 ORF4基因 |
Isolation of Strain BD and clone of ORF4 gene from porcine reproductive and respiratory syndrome virus |
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| Wang Jinfeng,Zhang Huimin,Yuan Wanzhe,Sun Jiguo.Isolation of Strain BD and clone of ORF4 gene from porcine reproductive and respiratory syndrome virus[J].Guangdong Journal of Animal and Veterinary Science,2011,36(3):33-35. |
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| Authors: | Wang Jinfeng Zhang Huimin Yuan Wanzhe Sun Jiguo |
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| Institution: | Wang Jinfeng,Zhang Huimin,Yuan Wanzhe,Sun Jiguo(College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | One porcine reproductive and respiratory syndrome virus strain BD was isolated from one pig of Hebei through cell culture.The full-length of ORF4 gene encoding the envelope glycoprotein(GP4) of PRRSV BD strain was amplified by RT-PCR with a pair of specific primers designed according to the relevant sequence of PRRSV JXA1 strain and then cloned into plasmid pMD19-T.The recombinant plasmid named as pMD-GP4 was subjected to sequencing and analysis.The sequence was compared with that of other PRRSV strains in ... |
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| Keywords: | Porcine reproductive and respiratory syndrome virus strain BD ORF4 gene |
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