| 大豆根际土壤真菌分子生物学鉴定方法 |
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| 引用本文： | 接伟光,张勇,蔡柏岩,白莉,王丽阳,李二平.大豆根际土壤真菌分子生物学鉴定方法[J].大豆科学,2011,30(3):384-387. |
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| 作者姓名： | 接伟光 张勇 蔡柏岩 白莉 王丽阳 李二平 |
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| 作者单位： | 黑龙江东方学院食品与环境工程学部;黑龙江大学生命科学学院分子生物学重点实验室;东北林业大学生命科学学院; |
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| 基金项目： | 黑龙江省教育厅2010年度科学技术研究(指导)项目(11553080); 黑龙江省自然科学基金资助项目(C200918); 黑龙江省博士后科研启动金资助项目(LBH-Q09022); 黑龙江大学高层次人才支持计划资助项目(生态修复团队Hdtd2010-12) |
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| 摘 要： | 以大豆根际土壤为研究对象,采用湿筛倾析-蔗糖离心法分离大豆根际土壤真菌孢子.应用Nested-PCR技术扩增其28S rDNA D1/D2区域,并结合DNA测序、系统发育分析,对其进行分类鉴定.结果表明:筛选出的大豆根际土壤真菌孢子DT-1与土壤真菌(AB438763)有较高的序列同源性,为97%;DT-2与Mucor...
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| 关 键 词： | 土壤 真菌 大豆 Nested-PCR |
Molecular Identification of the Fungi in the Rhizospheric Soil of Soybean |
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| JIE Wei-guang,ZHANG Yong,CAI Bai-yan,BAI Li,WANG Li-yang,LI Er-ping.Molecular Identification of the Fungi in the Rhizospheric Soil of Soybean[J].Soybean Science,2011,30(3):384-387. |
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| Authors: | JIE Wei-guang ZHANG Yong CAI Bai-yan BAI Li WANG Li-yang LI Er-ping |
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| Institution: | JIE Wei-guang1,ZHANG Yong1,CAI Bai-yan2,BAI Li1,WANG Li-yang2,LI Er-ping3 (1.Department of Food and Environment Engineering,Heilongjiang East University,Harbin 150086,Heilongjiang,2.Key Laboratory of Microbiology,College of Life Sciences,Heilongjiang University,Harbin 150080,3.College of Life Sciences,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | The fungi in the rhizospheric soil of soybean played a vital role in the formation of soil fertility,the spread of soil-borne diseases,the occurrence of allelopathy,etc.In particularly,soybean root rot caused by soil fungi has became a major disease which result in soybean production declined dramatically in China.Therefore,the rapid identification of the fungi in rhizospheric soil of soybean has great significance in preventing and curing fungal diseases of soybean.In this study,the fungal spores were isol... |
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| Keywords: | Soil Fungi Soybean Nested-PCR |
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