| 一株鸽新城疫病毒的分离鉴定 |
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| 引用本文： | 赵明秋,胡永明,许丽娜,易琳.一株鸽新城疫病毒的分离鉴定[J].广东畜牧兽医科技,2011,36(3):36-38. |
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| 作者姓名： | 赵明秋 胡永明 许丽娜 易琳 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 摘 要： | 对广东地区疑似发生鸽新城疫感染的鸽病料进行病毒分离,通过血凝试验(HA)、中和试验、F基因扩增及序列测定.结果分离到1株血凝效价为4log2,且能被NDV阳性血清中和的病毒;用针对NDV F基因设计的特异性鉴定引物对该分离株进行PCR扩增,可扩增出相应的目的片段;测序及Blast分析表 明其与山东分离株chicken/...
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| 关 键 词： | 新城疫病毒 分离 鉴定 鸽 广东 |
Isolation and identification of a Newcastle disease virus from pigeon |
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| Zhao Mingqiu,Hu Yongming,Xu Lina,Yi Lin.Isolation and identification of a Newcastle disease virus from pigeon[J].Guangdong Journal of Animal and Veterinary Science,2011,36(3):36-38. |
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| Authors: | Zhao Mingqiu Hu Yongming Xu Lina Yi Lin |
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| Institution: | Zhao Mingqiu,Hu Yongming,Xu Lina,Yi Lin(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | A Newcastle disease virus(NDV) was isolated from the pigeon in Guangdong.It was identified NDV with hemagglutination test,neutralization test,PCR and sequence analysis.The HA titer was 4㏒2 and this virus could be neutralized by the standard antibody of NDV.The F gene was amplified by RT-PCR by the specific identification primers of NDV F and sequenced.The homology of F gene was 95% with that of chicken/China/SD5/2008.Phylogenetic analysis showed that this virus was clustered into genotype Ⅵ.So this isolate was identified as NDV and named GD2010. |
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| Keywords: | Newcastle disease virus(NDV) isolation identification pigeon Guangdong |
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