Detection of antibodies to Cooperia ssp. and Ostertagia ssp. in calves with the enzyme linked immunosorbent assay (ELISA) |
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| Authors: | A Keus A Kloosterman R Van den Brink |
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| Institution: | Department of Animal Husbandry, Agricultural University, P.O. Box 338, 6700 AH Wageningen, The Netherlands |
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| Abstract: | The ELISA technique was found to be very useful for the detection and monitoring of anti-Cooperia and anti-Ostertagia antibodies in calves. Six differens antigens were used; saline extracts from third- and fourth-stage larvae and from adult worms of both genera.Some degree of genus specificity was found when using L4 or adult antigens but not when L3 antigens were used. Stage-specificity could be observed for Cooperia L4 antigen for a limited period after primary single infection. These findings were supported by the results of immuno-electrophoresis. |
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