| 3株新城疫病毒广西分离株L基因的克隆与序列分析 |
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| 引用本文： | 唐小飞,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤.3株新城疫病毒广西分离株L基因的克隆与序列分析[J].畜牧与兽医,2006,38(4):18-20. |
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| 作者姓名： | 唐小飞 谢芝勋 刘加波 庞耀珊 邓显文 谢志勤 |
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| 作者单位： | 广西兽医研究所,广西,南宁,530001 |
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| 基金项目： | 广西科技攻关项目(桂科攻0235001-4)，广西留学基金项目(桂科回0236005)，广西水产畜牧局科研计划资助 |
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| 摘 要： | 根据GenBank登陆的新城疫病毒L基因序列,设计了3对引物(L1和L2、L3和L4、L5和L6)。用RT-PCR技术对3株新城疫病毒广西分离GX7/02、GX9/03、GX11/03的L基因进行了分段扩增和克隆,并对克隆出来的3个片段进行序列测定,用DNAstar软件比较分析后进行拼接,得到长约为6.8 kb、包含有L基因全长的核苷酸序列。L基因的RNA全长为6 704 bp,拥有一个6 615 bp的开放阅读框,推导其编码的氨基酸数为2 204个。氨基酸同源性分析表明广西分离株之间同源性为98.6%~98.7%;与ZJ1株同源性为98.8%~98.9%;与La-Sota、B1、F48E9、HB92同源性为92.0%~94.2%。
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| 关 键 词： | 新城疫病毒 L基因 克隆 序列分析 |
| 文章编号： | 0529-5130（2006）04-0018-03 |
| 收稿时间： | 2005-09-11 |
| 修稿时间： | 2005-09-11 |
Cloning and sequence analysis of L gene of three Newcastle disease virus isolates in Guangxi |
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| TANG Xiao-fei,XIE Zhi-xun,LIU Jia-bo,PANG Yao-shan,DENG Xian-wen,XIE Zhi-qin.Cloning and sequence analysis of L gene of three Newcastle disease virus isolates in Guangxi[J].Animal Husbandry & Veterinary Medicine,2006,38(4):18-20. |
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| Authors: | TANG Xiao-fei XIE Zhi-xun LIU Jia-bo PANG Yao-shan DENG Xian-wen XIE Zhi-qin |
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| Institution: | Guangxi Veterinary Research Institute, Nanning 530001, China |
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| Abstract: | Three pair of primers(L1 and L2,L3 and L4,L5 and L6) were designed and synthesized according to the sequence of Newcastle disease virus from GenBank.L genes of GX7/02,GX9/03,GX11/03 NDV Guangxi isolates were amplified by RT-PCR using these primers.The PCR products were cloned and sequenced.Complete nucleotide sequence of L gene of these NDV isolates were obtained by splicing three fragments.The whole sequences of three L genes were analyzed using DNA Star software respectively.The results indicated that the L genes with 6704 bp long had a single opening read frame 6615 bp and coded a polypeptide of 2204 amino acids of these NDV isolates.The results of amino acid sequence comparison showed that the 2204 amino acids shared 98.6 to 98.7% of homogeneity in these Guangxi isolates,shared 98.8% to 98.9% with NDV ZJ1 strain,and 92.2% to 94.2% with NDV Lasota,B1,F48E9 and HB92. |
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| Keywords: | Newcastle disease virus L gene cloning sequencing |
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