| 家蚕耐热近等基因系的构建及分子标记育种技术的开发(摘要)(英文) |
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| 引用本文： | 赵远,吴阳春,张健.家蚕耐热近等基因系的构建及分子标记育种技术的开发(摘要)(英文)[J].农业科学与技术,2010,11(1):92-95. |
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| 作者姓名： | 赵远 吴阳春 张健 |
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| 作者单位： | 赵远,张健,ZHAO Yuan,ZHANG Jian(江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018);吴阳春,WU Yang-chun(中国农业科学院蚕业研究所,江苏镇江,212018) |
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| 基金项目： | 江苏高技术项目，江苏科技大学博士基金 |
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| 摘 要： | 目的]为家蚕近等基因系分子标记育种技术的开发利用提供理论依据。方法]采用构建近等基因系的方法创建新种质。利用分子标记辅助育种原理,采用连续回交方法将耐热基因导入到敏感品系欧17中。采用常规耐热选择结合分子标记辅助育种技术,经过6代回交、接种选择,2代自交,获得对具有耐热基因的家蚕新种质。结果]经过3年8代分别用欧17(敏感性品种)为父本,东34为母本(抗性品种)杂交得BC6F3,后以欧17为轮回亲本,育成了具有耐热基因的近等基因系NK(BC6F3)。同时监测了各回交后代的抗性水平,测定了近等基因系的分子标记,并利用SSR标记与耐热基因连锁的品种尝试耐热实用品种的杂交种组配。结论]成功构建了耐热近等基因系,监测了回交后代的抗性水平,饲养成绩,并测定了近等基因系的分子标记。
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| 关 键 词： | 蚕品种 培育 近等基因系 SSR分子标记 |
The Construction of Heat-resistant Near Isogenic Line (NIL) of Bombyx mori and the Development of Molecular Marker Breeding Technique |
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| ZHAO Yuan,WU Yang-chun,ZHANG Jian.The Construction of Heat-resistant Near Isogenic Line (NIL) of Bombyx mori and the Development of Molecular Marker Breeding Technique[J].Agricultural Science ＆ Technology,2010,11(1):92-95. |
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| Authors: | ZHAO Yuan WU Yang-chun ZHANG Jian |
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| Institution: | 1,21.Institute for Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang 212018; 2.Silk Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018 |
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| Abstract: | Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs. |
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| Keywords: | Silkworm species Cultivation Near-isogenic lines SSR molecular marker |
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