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| 辽宁省部分地区猪繁殖与呼吸综合征病毒流行株ORF5-7基因遗传变异分析 | |
| 引用本文： | 尹国友,吴发兴,郭爱英,李晓成,王洪斌,黄保续,张燕霞.辽宁省部分地区猪繁殖与呼吸综合征病毒流行株ORF5-7基因遗传变异分析[J].中国动物检疫,2007,24(3):25-27. |
| 作者姓名： | 尹国友 吴发兴 郭爱英 李晓成 王洪斌 黄保续 张燕霞 |
| 作者单位： | 1. 东北农业大学动物医学学院,黑龙江哈尔滨,150030;中国动物卫生与流行病学中心,山东青岛,266032 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 中国动物卫生与流行病学中心,山东青岛,266032;广西大学动物科技学院,广西南宁,530004 4. 东北农业大学动物医学学院,黑龙江哈尔滨,150030 |
| 摘 要： | 根据GenBank发表的猪繁殖与呼吸综合征病毒(PRRSV)ATCC VR-2332全基因序列,设计并合成2对引物,采用RT-PCR技术,对辽宁省站送检的病料进行分离扩增,分别获得相应的目的片段,将其分别克隆入pMD18-T载体中,并送上海生工测序。应用DNAStar软件分析所测序列,并与ATCC VR-2332、CH-1a、BJ-4、LV-M96262及疫苗株的ORF567基因进行序列比较,通过核苷酸序列分析表明这5株流行株与VR-2332、CH-1a、BJ-4等代表株的同源性为89.5%～95%,与LV-M96262的同源性为54%～58.3%.氨基酸序列分析表明这5株流行株与VR-2332、CH-1a、BJ-4等代表株的同源性为82.8%～94.7%. |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF567基因 序列分析 |
| 文章编号： | 1005-944X（2007）03-0025-03 |
Analysis in Heredity and Variation of the Open Reading Frame ORF567 Genome Sequence of Prevalence Strains of Porcine Reproductive and Respiratory Syndrome Virus from Liaoning Region |
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| Yin Guoyou,Wu Faxing,Guo Aiying,Li Xiaocheng,Wang Hongbin,Huang Baoxu,Zhang Yanxia.Analysis in Heredity and Variation of the Open Reading Frame ORF567 Genome Sequence of Prevalence Strains of Porcine Reproductive and Respiratory Syndrome Virus from Liaoning Region[J].China Journal Of Animal Quarantine,2007,24(3):25-27. | |
| Authors: | Yin Guoyou Wu Faxing Guo Aiying Li Xiaocheng Wang Hongbin Huang Baoxu Zhang Yanxia |
| Abstract: | According to the sequence of ATCC VR-2332 strain of porcine reproductive and respiratory syndrome virus published by the Genbank, two pairs of primers were designed for RT-PCR amplification of PRRSV isolates from Liaoning. The corresponding DNA fragments were abtained and , cloned into pMD18-T vector respectively and sequenced in Shanghai. The sequences of ORF567 genes were analysed using DNAStar and compared with those of ATCC VR-2332,CH-1a, BJ-4 LV-M96262 and vaccine strains. Nuclear acid analysis indicates that the homology was up to 89.5%-95% between Liaoning strains and VR-2332,CH-la, B J-4, and 54%-58.3% between Liaoning strains and LV-M96262. Amino acid analysis indicates that the ho- mology was up to 82.8%-94.7% between Liaoning strains and VR-2332,CH-1a,BJ-4 . |
| Keywords: | porcine reproductive and respiratory syndrome virus ORF_(567) gene sequence analysis |
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