| 中国不同纬度野生大豆和栽培大豆SSR分析 |
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| 引用本文： | 赵洪锟,王玉民,李启云,张明,庄炳昌.中国不同纬度野生大豆和栽培大豆SSR分析[J].大豆科学,2001,20(3):172-176. |
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| 作者姓名： | 赵洪锟 王玉民 李启云 张明 庄炳昌 |
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| 作者单位： | 吉林省农业科学院 |
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| 基金项目： | 国家自然科学基金重点项目 (基础上编号 :39730 330，396 70 448)，“973”资助项目 |
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| 摘 要： | 采用SSR分子标记技术对我国不同纬度的野生大豆（G.soja)和栽培大豆（G.max)各22份进行了多样性分析，通过对所合成的40对引物的筛选，12对引物扩增结果表明出良好的多态性，引物BARC-sat39在野生大豆和栽培大豆之间有特异谱带，表明这个SSR标记是与栽培大豆和野生大豆有关的一个等位基因位点，通过对实验结果量化后数据分析；野生大豆和栽培大豆的平均遗传距离分别为0.175和0.150，表明野生大豆的多态性比栽培大豆较为丰富，在遗传距离0.300处，野生大豆和栽培大豆被明显分为二类，与以往大豆属Soja亚属的形态学分类结果相一致，为野生大豆和栽培大豆分为二个种提供了分子水平上的证据。
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| 关 键 词： | SSR 野生大豆 栽培大豆 遗传多样性 中国 纬度 |
| 文章编号： | 1000-9841(2001)03-0172-05 |
SSR ANALYSIS OF WILD SOYBEAN ( G. soja ) AND CULTIVATED SOYBEAN FROM DIFFERENT LATITUDE IN CHINA |
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| Zhao Hongkun,Wang Yumin,Li Qiyun,Zhang Ming,Zhuang Bingchang.SSR ANALYSIS OF WILD SOYBEAN ( G. soja ) AND CULTIVATED SOYBEAN FROM DIFFERENT LATITUDE IN CHINA[J].Soybean Science,2001,20(3):172-176. |
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| Authors: | Zhao Hongkun Wang Yumin Li Qiyun Zhang Ming Zhuang Bingchang |
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| Abstract: | DNA genetic variation of 22 accessions of wild soybean and 22 accessions of cultivated soybean, which were from different latitude in China, was studied by SSR technique. Forty pairs of soybean SSR primers were synthesized according to published data, and 12 out of them had polymorphic alleles. Specific bands were amplified by primer 3 between wild soybean and cultivated soybean, which showed this SSR marker was one of the alleles among wild soybean and cultivated soybean. The data analysis showed that: The average genetic distance of wild soybean and cultivated soybean were 0.176 and 0.150 respectively which agreed on the thesis that wild soybean had more polymorphism than that of cultivated soybean. And also it provided a molecular proof that wild soybean and cultivated soybean belonged to two species from the dendgrom at the site of genetic distance 0.300, which conformed to phenotypic results. |
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| Keywords: | SSR Wild soybean (G soja) Cultivated soybean (G max) Genetic diversity |
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