| 小麦未减数配子基因的连锁标记及染色体区段检测 |
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| 引用本文： | 寇春兰,赵来宾,刘梦,郝明,甯顺腙,袁中伟,刘登才,张连全.小麦未减数配子基因的连锁标记及染色体区段检测[J].作物学报,2016,42(7):984-989. |
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| 作者姓名： | 寇春兰 赵来宾 刘梦 郝明 甯顺腙 袁中伟 刘登才 张连全 |
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| 作者单位： | 四川农业大学小麦研究所四川成都 611130 |
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| 基金项目： | 本研究由国家自然科学基金项目(31271723, 31201210), 四川省杰出青年基金项目(2011JQ0016)和四川省教育厅科研项目(14ZA0012)资助。 |
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| 摘 要： | 六倍体普通小麦(Triticumaestivum L., AABBDD, 2n=42)由四倍体小麦(T. turgidum, AABB, 2n=28)与节节麦(Aegilops tauschii Cosson, DD, 2n=14)天然杂交，然后通过染色体自动加倍形成。加倍过程主要受四倍体小麦未减数配子基因控制，且不同四倍体小麦存在不同的遗传效应。本研究利用位于3B染色体上未减数配子基因QTug.sau-3B的连锁SSR标记Xgpw1146和高通量DArTseq分子标记，筛选出可能转入四倍体小麦未减数配子基因的人工合成小麦改良后代。在105份改良材料中检测出17份具有四倍体小麦的Xgpw1146等位位点，表明四倍体小麦的未减数配子基因可能转入了这17份材料。利用DArTseq高通量标记技术分析人工合成小麦SHW-L1的88份改良后代，发现含四倍体小麦Xgpw1146等位位点的材料均具有来自SHW-L1、且可能包含Xgpw1146的一个染色体区段，表明未减数配子基因临近区域以一个区段传递到改良后代。这些人工合成小麦改良材料在加倍单倍体育种中有重要的应用潜力。
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| 关 键 词： | 异源多倍体 人工合成小麦 未减数配子 |
| 收稿时间： | 2015-11-04 |
Detection of the Molecular Marker and Chromosomal Segment linked to Unreduced Gamete Gene in Common Wheat |
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| KOU Chun-Lan,ZHAO Lai-Bin,LIU Meng,HAO Ming,NING Shun-Zong,YUAN Zhong-Wei,LIU Deng-Cai,ZHANG Lian-Quan.Detection of the Molecular Marker and Chromosomal Segment linked to Unreduced Gamete Gene in Common Wheat[J].Acta Agronomica Sinica,2016,42(7):984-989. |
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| Authors: | KOU Chun-Lan ZHAO Lai-Bin LIU Meng HAO Ming NING Shun-Zong YUAN Zhong-Wei LIU Deng-Cai ZHANG Lian-Quan |
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| Institution: | Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China |
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| Abstract: | Hexaploid common wheat (Triticum aestivum L., AABBDD, 2n=42) arose fromspontaneous chromosome doubling of the hybrid between T. turgidum and Aegilops tauschii Cosson. The process of chromosomes doubling is mainly determined by unreduced gametes (UG)genes in T. turgidum. The genetic effects on the UG production may vary among T. turgidum lines. In this study, a SSR marker close to the UG geneQTug.sau-3B(Xgpw1146) and high throughput DArTseq genotyping technique were used to screen the UG gene in common wheat lines transferred from T. turgidum via synthetic hexaploid wheat (SHW) as a bridge. Out of the analyzed 105 SHW-derived elite lines, 17 had the Xgpw1146allele from T. turgidum, indicating that the UG gene was probably transferred into these wheat lines. According to the DArTseq genotyping data on 88 lines derived from the synthetic hexaploid wheat SHW-L1, all these lines with the T. turgidumXgpw1146allele contained a chromosomal segment of SHW-L1,probably covering the Xgpw1146 locus. This indicatesthat the adjacent region of the UG gene as a chromosomal segment was transferred into wheat lines. These SHW-derived lines have important application potential on wheat doubled haploid breeding. |
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| Keywords: | Allohexaploid Synthetic wheat Unreduced gamete |
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