| 一个水稻黄绿叶突变基因的定位和遗传研究 |
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| 引用本文： | 初志战,郭海滨,刘小林,陈远玲,刘耀光.一个水稻黄绿叶突变基因的定位和遗传研究[J].作物学报,2016,42(5):684-689. |
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| 作者姓名： | 初志战 郭海滨 刘小林 陈远玲 刘耀光 |
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| 作者单位： | 1.华南农业大学生命科学学院 / 亚热带农业生物资源保护与利用重点实验室, 广东广州 510642;2.华南农业大学公共基础课实验教学中心, 广东广州 510642;3.宜春学院, 江西宜春 336000 |
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| 基金项目： | 本研究由亚热带农业生物资源保护与利用国家重点实验室开放课题(SKL-CUSAb-2013-04)，江西省教育厅科技计划项目(GJJ14707)和广东省自然科学基金-博士启动项目(2015A030310485)资助。 |
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| 摘 要： | 从粳稻品种日本晴经60Co诱变的M1材料中发现一个黄绿叶突变体, 其叶片从萌发到三叶前期表现白化, 三叶后期开始转为黄绿叶, 直到衰老。遗传分析表明, 该突变表型受一对隐性核基因控制, 将该黄绿叶突变体暂定名为ygl8951。与野生型相比, ygl8951的叶绿素含量与类胡萝卜素含量显著降低。电子显微镜观察表明ygl8951内叶绿体数量明显减少, 叶绿体内没有基粒类囊体, 只有类似间质类囊体结构。基因表达定量分析表明, 突变体中光系统I和光系统II基因表达水平明显下调, 核糖体结构基因和质体编码的RNA聚合酶亚基基因表达明显上调。利用ygl8951与籼稻品种黄华占杂交获得的F2分离群体, 将该基因定位于水稻第6染色体上的In/Del标记607489与607611之间, 物理距离191 kb的范围内, 通过分析确认该基因为一个新的调控叶色的基因。
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| 关 键 词： | 水稻 黄绿叶 基因定位 遗传分析 |
| 收稿时间： | 2015-09-25 |
Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant in Rice |
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| CHU Zhi-Zhan,GUO Hai-Bin,LIU Xiao-Lin,CHEN Yuan-Ling,LIU Yao-Guang.Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant in Rice[J].Acta Agronomica Sinica,2016,42(5):684-689. |
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| Authors: | CHU Zhi-Zhan GUO Hai-Bin LIU Xiao-Lin CHEN Yuan-Ling LIU Yao-Guang |
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| Institution: | 1.College of Life Sciences, South China Agricultural University / State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China;2.Center of Experimental Teaching for Common Basic Course, South China Agricultural University, Guangzhou 510642, China;3.Yichun University, Yichun 336000, China |
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| Abstract: | A yellow-green leaf rice mutant, temporarily named as ygl8951 (yellow-green leaf 8951), was identified from 60Co γ-ray radiation mutation in japonica rice varietyNipponbare. The mutant showed albino phenotype from germination to 3-leaf-stage, then turned yellow-green phenotype till apoptosis. The contents of chlorophyll and carotenoid were obviously decreased in ygl8951 compared with the wild type. Electron microscope observation showed that no grana thylakoids but some stroma thylakoids-like structures were found in chloroplast of ygl8951 mutant. The expression levels of some genes involved in photosystem Iand photosystem II were dramatically decreased, while the ribosomal and RNA polymerase genes in chloroplast were increased in ygl8951 mutant compared with the wild type. Mapping-based cloning was used to identify the ygl8951 locus using an F2 population from a crossing between the mutant and Huanghuazhan. The result showed that the mutated locus was located in a 191 kb region on chromosome 6, which was assumed to be a new gene controlling leaf color. |
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| Keywords: | Rice Yellow-green leaf Gene mapping Genetic analysis |
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