| 水稻白穗突变体wp4的鉴定与基因精细定位 |
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| 引用本文： | 王晓雯,蒋钰东,廖红香,杨波,邹帅宇,朱小燕,何光华,桑贤春.水稻白穗突变体wp4的鉴定与基因精细定位[J].作物学报,2015,41(6):838-844. |
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| 作者姓名： | 王晓雯 蒋钰东 廖红香 杨波 邹帅宇 朱小燕 何光华 桑贤春 |
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| 作者单位： | 西南大学水稻研究所 / 转基因植物与安全控制重庆市重点实验室 / 南方山地农业教育部工程研究中心, 重庆 400716 |
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| 基金项目： | 本研究由中央高校基本科研业务费(XDJK2011c016)和国家自然科学基金项目(31171178)资助。 |
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| 摘 要： | 水稻开花灌浆期,内外颖呈绿色,含光合色素,为阐释非叶片组织叶绿体发育的分子机制,本文对EMS诱变获得的新型白穗突变体wp4进行了研究。抽穗灌浆期,wp4的穗轴呈绿色,内外颖呈乳白色;内外颖叶绿体发育不健全,无类囊体结构,叶绿素a、叶绿素b和类胡萝卜素含量极显著降低。此外,wp4全生育期叶片呈淡黄色,叶绿体内基质片层较少且排列松散;与野生型相比,wp4的叶绿素a含量显著降低,叶绿素b和类胡萝卜素含量虽略低,但差异未达到显著水平。农艺性状分析发现,与野生型相比,wp4的有效穗和结实率略有增加,其他性状则略有降低,但变化均未达到显著差异水平。遗传分析表明wp4受一对隐性核基因调控,利用1200株西农1A/wp4的F2隐性群体,最终将WP4精细定位在第8染色体约79 kb的物理范围内,根据水稻基因组注释计划,该区间包含14个基因,这为WP4的功能和作用机制研究奠定了基础,也为水稻标记性状育种提供了新的资源。
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| 关 键 词： | 水稻(Oryza sativa) 白穗黄绿叶 基因定位 叶色标记 |
| 收稿时间： | 2014-11-24 |
Identification and Gene Fine Mapping of White Panicle Mutant wp4 in Oryza sativa |
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| WANG Xiao-Wen,JIANG Yu-Dong,LIAO Hong-Xiang,YANG Bo,ZOU Shuai-Yu,ZHU Xiao-Yan,HE Guang-Hua,SANG Xian-Chun.Identification and Gene Fine Mapping of White Panicle Mutant wp4 in Oryza sativa[J].Acta Agronomica Sinica,2015,41(6):838-844. |
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| Authors: | WANG Xiao-Wen JIANG Yu-Dong LIAO Hong-Xiang YANG Bo ZOU Shuai-Yu ZHU Xiao-Yan HE Guang-Hua SANG Xian-Chun |
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| Institution: | Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China |
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| Abstract: | At the flowering and filling stages, rice hull appears green color and possesses photosynthetic pigment. To clear the mechanism of chloroplast development in the panicle, we identified a novel white panicle mutant from the progeny of indica restorer line Jinhui 10 with seeds treated by EMS and termed it as wp4. The wp4 displayed green spike-stalks and milk-white hulls after the heading stage. The structures of chloroplast and thylakoid were severely destroyed and the contents of photosynthetic pigment decreased extremely significantly in the mutational hulls. Compared with the wild type, the wp4 displayed yellow green leaves and contained looser stromal lamellae. And the contents of chlorophyll a, b, and carotenoid were all declined while only the changing of chlorophyll a led to the significantly different level in statistics. Except for effective panicles number and seed setting rate, other detected agronomic characteristics decreased slightly but the changing did not come up to the statistically significant difference. Genetic analysis indicated that the white panicle of wp4 was controlled by a recessive nuclear gene and which was finally mapped on chromosome 8 with 79 kb physical distances according to 1200 mutational plants derived from the F2 generation of Xinong1A/wp4. The restricted region contained 14 annotated genes based on Rice Genome Annotation Project. These results provided a foundation for gene cloning and function analysis of the WP4. Meanwhile, the traits of wp4 could be available in rice breeding as a morphological marker. |
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| Keywords: | Rice (Oryza sativa)White panicle Yellow green leaf Gene mapping Leaf color marker |
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