| 小麦全基因组NBS类R基因分析及2AL染色体NBS-SSR特异标记开发 |
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| 引用本文： | 乔麟轶,常建忠,郭慧娟,高建刚,郑军,畅志坚.小麦全基因组NBS类R基因分析及2AL染色体NBS-SSR特异标记开发[J].作物学报,2016,42(6):795-802. |
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| 作者姓名： | 乔麟轶 常建忠 郭慧娟 高建刚 郑军 畅志坚 |
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| 作者单位： | 1山西大学研究生院, 山西太原 030006; 2山西省农业科学院作物科学研究所 / 作物遗传与分子改良山西省重点实验室, 山西太原030031; 3山西省农业科学院小麦研究所, 山西临汾 041000; 4山西省农业科学院旱地农业研究中心, 山西太原030031; 5北京市农林科学院杂交小麦工程技术研究中心, 北京 100097 |
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| 基金项目： | 本研究由国家自然科学基金项目(31171839, 31401385), 山西省青年基金项目(2015021145), 山西省农业攻关项目(20150311001-1, 20150311001-5), 山西省农业科学院攻关项目(15YGG01)和北京市农林科学院青年基金项目(QNJJ201428)资助。 |
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| 摘 要： | NBS(nucleotide binding site)类基因是植物界中最重要的一类抗病基因。用信息学方法从普通小麦(Triticum aestivum L.)全基因组中分离出2406条含有NBS结构的完整蛋白序列,每条包含48~2272个氨基酸残基。根据NBS结构域两端是否连接CC或LRR结构域,将TaNBS分为N、CN、NL和CNL 4类。对TaNBS所在scaffold序列的SSR位点进行诊断,从1203条scaffold序列上发现2177个SSR位点,以二碱基重复位点最多,占73.5%。针对小麦2AL染色体上的51个SSR位点开发标记,缺体–四体和双端体验证结果表明,有39个标记(76.5%)为2AL特异标记,其中24个特异标记在抗白粉病材料Khapli(2AL上携带Pm4a)和感病材料Chancellor间存在多态性。利用近等基因系Khapli/8*Cc筛选出3个可能与Pm4a连锁的NBS-SSR标记,分别是Sxaas_2AL22、Sxaas_2AL39和Sxaas_2AL46。本研究开发的与抗病序列紧密连锁的特异SSR标记可用于2AL染色体上抗病新基因的检测以及已有抗病基因的候选序列筛选。
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| 关 键 词： | 小麦 抗病 NBS类基因 SSR标记 2AL染色体 |
| 收稿时间： | 2015-10-20 |
Genome-Wide Analysis of TaNBS Resistance Genes and Development of Chromosome 2AL-specific NBS-SSR Markers in Wheat |
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| QIAO Lin-Yi,CHANG Jian-Zhong,GUO Hui-Juan,GAO Jian-Gang,ZHENG Jun,CHANG Zhi-Jian.Genome-Wide Analysis of TaNBS Resistance Genes and Development of Chromosome 2AL-specific NBS-SSR Markers in Wheat[J].Acta Agronomica Sinica,2016,42(6):795-802. |
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| Authors: | QIAO Lin-Yi CHANG Jian-Zhong GUO Hui-Juan GAO Jian-Gang ZHENG Jun CHANG Zhi-Jian |
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| Abstract: | Nucleotide binding site (NBS)-encoding genes are the most important resistance genes (R genes) in plant kingdom. In this study, 2406 full-length NBS protein sequences, of which single protein varies from 48 aa to 2272 aa, were identified from wheat (Triticum aestivum L.) genome using bioinformatic method. These TaNBSs were divided into four categories, including N, CN, NL and CNL, according to whether the NBS domains connect CC or LRR domains at both ends. Diagnosis results showed that 1203 of all the scaffolds with TaNBS contained 2177 simple sequence repeats (SSR) loci, 73.5% of which were dinucleotide repeat sites. Based on the NBS-SSR loci on chromosome 2AL in wheat, we developed 51 molecular markers , and 39 of them (76.5%) were confirmed as chromosome specific markers using Chinese Spring nulli-tetrasomic and ditelosomic lines. Furthermore, 24 2AL-specific NBS-SSR markers showed polymorphism between resistant material Khapli carried Pm4a on chromosome 2AL and susceptible material Chancellor. Finally, three 2AL-specific NBS-SSR markers, Sxaas_2AL22, Sxaas_2AL39, and Sxaas_2AL46, were probably linked to Pm4a gene based on genetic linkage test using Pm4a-NILs (Khapli/8*Cc). These chromosome specific 2AL-NBS-SSR markers can be used to locate novel R genes or screen the candidate sequences for known R genes on chromosome 2AL. |
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| Keywords: | Wheat Disease resistance NBS-encoding genes SSR markers Chromosome 2AL |
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