| 水稻叶片早衰突变体ospls3的生理特征和基因定位 |
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| 引用本文： | 龚盼,黎坤瑜,黄福灯,韦荔全,杨茜,程方民,潘刚.水稻叶片早衰突变体ospls3的生理特征和基因定位[J].作物学报,2016,42(5):667-674. |
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| 作者姓名： | 龚盼 黎坤瑜 黄福灯 韦荔全 杨茜 程方民 潘刚 |
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| 作者单位： | 1.浙江大学农业与生物技术学院, 浙江杭州310058;2.浙江省农业科学院, 浙江杭州310021 |
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| 基金项目： | 本研究由国家自然科学基金项目(31271691)和国家转基因生物新品种培育重大专项(2013ZX08001-002)资助。 |
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| 摘 要： | 叶片衰老是作物叶片发育的最后阶段，功能叶早衰将影响作物产量和品质，因此，研究叶片早衰的分子与生理机制对于培育耐早衰优良品种具有重要意义。本研究利用60Co辐射诱变籼稻N142，获得叶片早衰突变体ospls3，其叶片早衰始于分蘖期，最先表现为叶尖变褐及叶中上部出现褐色斑点，并向叶基部蔓延而使叶片枯死。生理分析表明，野生型剑叶的叶绿素含量显著低于倒二叶和倒三叶，而突变体的含量则分别低于野生型且依次显著降低；野生型剑叶、倒二叶和倒三叶间的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、O2?含量和H2O2含量基本不变，而突变体的这些活性和含量则依次显著升高；野生型剑叶、倒二叶和倒三叶的可溶性蛋白含量和过氧化氢酶(CAT)活性变化不显著，而突变体则依次降低。遗传分析表明，ospls3受1对隐性基因控制，借助图位克隆技术将该基因定位于第12染色体长臂的RM6953与RM28753之间，物理距离为294 kb，该结果为进一步克隆OsPLS3基因并研究其功能奠定了基础。
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| 关 键 词： | 水稻 ospls3 叶片早衰 生理分析 基因定位 |
| 收稿时间： | 2015-09-12 |
Physiological Characteristics and Gene Mapping of a Precocious Leaf Senescence Mutant ospls3 in Rice |
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| GONG Pan,LI Kun-Yu,HUANG Fu-Deng,WEI Li-Quan,YANG Xi,CHENG Fang-Min,PAN Gang.Physiological Characteristics and Gene Mapping of a Precocious Leaf Senescence Mutant ospls3 in Rice[J].Acta Agronomica Sinica,2016,42(5):667-674. |
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| Authors: | GONG Pan LI Kun-Yu HUANG Fu-Deng WEI Li-Quan YANG Xi CHENG Fang-Min PAN Gang |
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| Institution: | 1.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;2.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | Leaf senescence is the final stage of leaf development. However, premature aging of functional leaves leads to yield reduction and quality decline. Thus, it is very important for developing novel crop germplasms with delayed leaf-senescence characteristics through investigating the molecular mechanism of leaf senescence. In this study, an ospls3 (Oryza sativa precocious leaf senescence 3) mutant, produced by 60Co γ-radiation treatment of indica cultivar N142, was identified. The symptoms of the premature senescence mutant presented firstly at tillering stage showing brown leaf tip and brown spots in top part of leaf blade, then spread rapidly to basal part of leaf blade and led leaf to die. The physiological analysis indicated that, in the ospls3 mutant, the content of chlorophyll was the highest in the flag leaf, the following was in second-top and third-top leaves, but all of them were significantly lower than those in the wild type. The contents of MDA, O2?, and H2O2 and the activities of SOD and POD among these top three leaves in the wild type maintained similar levels, which were significantly lower than those in the mutant type. The soluble protein contents and the activity of CAT had no significant difference among top three leaves in the wild type while significantly decreased in the mutant. Genetic analysis verified that the ospls3 is a recessive mutant and was mapped in a 294 kb interval between RM6953 and RM28753 on the long arm of chromosome 12, which establishes a solid foundation for further cloning and functional studies of this gene. |
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| Keywords: | Rice ospls3 Precocious leaf senescence Physiological analysis Gene mapping |
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