| 表面等离子共振生物传感技术测定蜂蜜中残留的泰乐菌素 |
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| 引用本文： | 李熠,周金慧,赵静,薛晓锋,陈芳,吴黎明.表面等离子共振生物传感技术测定蜂蜜中残留的泰乐菌素[J].中国养蜂,2008,59(1):11-13. |
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| 作者姓名： | 李熠 周金慧 赵静 薛晓锋 陈芳 吴黎明 |
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| 作者单位： | 中国农业科学院蜜蜂所农业部蜂产品质量监督检验测试中心 北京100093 |
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| 摘 要： | 本文利用表面等离子共振生物传感器技术对蜂蜜样品中残留的泰乐菌素进行测定。蜂蜜样品通过缓冲溶液提取后在BIAcoreQ大分子互作仪上测定,并且与试剂盒方法进行对比。结果表明采用表面等离子共振生物传感器方法在0.5-10ng/g的线性范围内,检出限为0.5ng/g,平均回收率在90.3%-101.7%之间,日内变异系数和日间变异系数分别为2.1%-4.7%和3.9%-7.1%(n=6),准确度偏离均小于10%。
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| 关 键 词： | 表面等离子共振传感器 蜂蜜 泰乐菌素 |
| 文章编号： | 0412-4367（2008）01-0011-03 |
Determination of Tylosin Residue in Honey by Surface Plasmon Resonance(SPR) |
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| Li Yi,Zhou Jinhui,Zhao Jing,Xue Xiaofeng,Chen Fang,Wu Liming.Determination of Tylosin Residue in Honey by Surface Plasmon Resonance(SPR)[J].Apiculture of China,2008,59(1):11-13. |
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| Authors: | Li Yi Zhou Jinhui Zhao Jing Xue Xiaofeng Chen Fang Wu Liming |
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| Abstract: | In this study, trace of tylosin residue in honey was determined by Surface Plasmon Resonance. Prior to analysis in BIAcore 3000, tylosin was only extracted from honey samples using HBS-EP buffer. The standard calibration curve for tylosin was constructed with a good linearity (R2>0.99) in the concentration range of 0.5~10 ng/g. The limit of detection was 0.5 ng/g for tylosin in honey. Average recoveries ranged from 90.3%~101.7%. The intra- and inter-assay CVs were 2.1%~4.7% and 3.9%~7.1%, respectively(n=6), whereas accuracy, which has been calculated as a percentage of the real concentration, never deviated from 100% by more than 10%. |
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| Keywords: | Surface Plasmon Resonance (SPR) Honey Tylosin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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