| 钙稳态失衡在锰致体外培养鸡胚脑神经细胞凋亡中的作用 |
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| 引用本文： | 陈蕾,李志鹏,李术,徐世文.钙稳态失衡在锰致体外培养鸡胚脑神经细胞凋亡中的作用[J].畜牧兽医学报,2010,41(3). |
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| 作者姓名： | 陈蕾 李志鹏 李术 徐世文 |
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| 作者单位： | 1. 东北农业大学动物医学学院,哈尔滨,150030
2. 东北农业大学动物医学学院,哈尔滨,150030;山东省安丘市凌河镇畜牧兽医管理站,安丘,262100 |
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| 摘 要： | 旨在探讨钙稳态失衡在锰致体外培养鸡胚脑神经细胞凋亡中的作用。以体外培养鸡胚脑神经元为研究对象,在含终浓度为0、1.5、22、.5 mmol.L-1MnCl2的DMEM培养液中培养24 h,应用流式细胞仪检测磷脂酰丝氨酸(PS)和线粒体膜电位(ΔΨm),琼脂糖凝胶电泳法(DNA Ladder)检测细胞染色质的断裂,以Fura-3/AM为探针检测细胞内游离钙离子浓度(Ca2+]i),用实时定量PCR法检测细胞内CaMmRNA的表达水平。结果表明,随着MnCl2浓度的升高,ΔΨm呈降低趋势,PS膜外翻增加,细胞染色质DNA发生损伤,产生明显的梯形条带,细胞内Ca2+]i呈升高趋势,CaMmRNA的表达水平下降。结果提示,过量锰通过抑制CaM活性,引起神经元内Ca2+]i升高,膜通透性发生改变,ΔΨm降低,从而导致鸡胚脑神经元发生凋亡。
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| 关 键 词： | 钙稳态 锰 鸡 神经细胞 细胞凋亡 |
Effect of the Disequilibrium of Calcium Homeostasis on the Apoptosis of the Neurons in the Chicken Brain Induced by Manganese in Vitro |
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| CHEN Lei,LI Zhi-peng,LI Shu,XU Shi-wen.Effect of the Disequilibrium of Calcium Homeostasis on the Apoptosis of the Neurons in the Chicken Brain Induced by Manganese in Vitro[J].Acta Veterinaria et Zootechnica Sinica,2010,41(3). |
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| Authors: | CHEN Lei LI Zhi-peng LI Shu XU Shi-wen |
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| Institution: | CHEN Lei1,LI Zhi-peng1,2,LI Shu1,XU Shi-wen1(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China,2.Linghe Animal Husb,ry , Veterinary Station,Anqiu 262100,China) |
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| Abstract: | To study the effect of the disequilibrium of calcium homeostasis on the apoptosis of the neurons in the chicken brain induced by manganese in vitro , neurons cultured in vitro were chosen as the research object. Neurons were cultured in the DMEM for 24 h with the final concentration of 0, 1. 5, 2 and 2. 5 mmol·L~(-1) MnCl_2. The mitochondrial membrane potential (ΔΨm) and phosphatidylserine (PS) were assessed by flow cytometry. DNA fragmentations were detected by DNA Ladder. The calcium ion concentration within neurons (Ca~(2+)]i) was detected using the Fura3/AM as the probe and expression level of intracellular CaM mRNA was detected by FQPCR. The results showed that ΔΨm tended to decline the expansion of the pore, DNA fragmentations and Ca~(2+)]i were increased. Moreover, the expression of CaM mRNA was decreased.This study indicated that MnCl_2 could decrease the activity of CaM, ΔΨm and increase Ca~(2+)] result in apoptosis of the chicken neurons. |
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| Keywords: | calcium homeostasis manganese chicken neurons apoptosis |
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