| 鸡大肠杆菌和奇异变形杆菌的分离、鉴定及药敏分析 |
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| 引用本文： | 潘玉善,苑丽,刘建华,胡功政,莫娟.鸡大肠杆菌和奇异变形杆菌的分离、鉴定及药敏分析[J].江西农业学报,2009,21(12):156-159. |
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| 作者姓名： | 潘玉善 苑丽 刘建华 胡功政 莫娟 |
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| 作者单位： | 河南农业大学,牧医工程学院,河南,郑州,450002 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 采用全自动微生物鉴定系统(VITEK-32)鉴定了一例临床分离鸡致病菌,并进行了超广谱β-内酰胺酶(ES-BLs)的检测和用肉汤微量稀释法测定12种药物和4种复方药物对分离菌的抗菌活性。结果显示,分离的4株菌中鉴定为大肠埃希氏菌3株、奇异变形杆菌1株,在分离的4株菌中均产ESBLs。药敏试验结果表明4株菌对氨苄西林、头孢噻肟、头孢噻呋、头孢曲松除一株中介外其它均耐药,对阿米卡星均敏感,尤其1株奇异变形杆菌和1株大肠杆菌耐药严重,对12种药物中有10种耐药,显示出严重的多重耐药性。β-内酰胺类药物与酶抑制剂联用能使药物对细菌的最低抑菌浓度(M ICs)降低4~64倍,因此复方β-内酰胺类药物仍是防治鸡致病菌感染的有效措施之一。
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| 关 键 词： | 鸡 大肠杆菌 奇异变形杆菌 超广谱β-内酰胺酶(ESBLs) 药敏试验 |
Isolation, Identification and Antimicrobial Susceptibility of Escherichia coli and Proteus mirabilis Isolated from Chicken |
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| PAN Yu-shan,YUAN Li,LIU Jian-hua,HU Gong-zheng,MO Juan.Isolation, Identification and Antimicrobial Susceptibility of Escherichia coli and Proteus mirabilis Isolated from Chicken[J].Acta Agriculturae Jiangxi,2009,21(12):156-159. |
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| Authors: | PAN Yu-shan YUAN Li LIU Jian-hua HU Gong-zheng MO Juan |
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| Abstract: | The bacterial strains were isolated from chicken and identified by the VITEK-32 system.The extended-spectrum β-lactamases(ESBLs) were detected by disk diffusion method and antimicrobial susceptibility testing was performed by broth micro-dilution assay using 12 kinds of drugs and 4 kinds of compound drugs.The results revealed that the bacterial strains were identified as Escherichia coli and Proteus mirabilis.The 4 clinically isolated strains could produce ESBLs.The results of antimicrobial susceptibility testing showed that the 3 Escherichia coli strains and 1 Proteus mirabilis strain were all resistant to ampicillin,cefotaxime,ceftiofur and ceftriaxone except 1 Proteus mirabilis strain was intermediate to cefotaxime;all strains were susceptible to amikaxin;and 1 Escherichia coli strain and 1 Proteus mirabilis strain isolated from the same diseased chicken were resistant to 10 of 12 kinds of drugs especially.This result demonstrated the serious multi-drug resistance.The minimum inhibitory concentration(MICs) of β-lactam antibiotics combined with enzyme inhibitor against Escherichia coli strains and Proteus mirabilis decreased by 4~32 and 8~64 times,respectively.Thus the application of compound β-lactam drugs is one of effective measures for the prevention and treatment of chickens infected with the bacteriosis. |
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| Keywords: | Escherichia coli Proteus mirabilis Extended-spectrum β-lactamases Antimicrobial susceptibility testing |
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