| 蜡梅花被片mRNA差异显示分析体系的建立与优化 |
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| 引用本文： | 周明芹,王波,文璐,陈龙清.蜡梅花被片mRNA差异显示分析体系的建立与优化[J].湖北农业科学,2008,47(1):8-11. |
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| 作者姓名： | 周明芹 王波 文璐 陈龙清 |
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| 作者单位： | 华中农业大学园艺植物生物学教育部重点实验室,武汉,430070 |
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| 摘 要： | 以同一单株狗蝇蜡梅的中被片、内被片为材料,首次将mRNA差异显示技术用于蜡梅花被片的研究.建立了蜡梅优化的mRNA差异显示分析体系,并获得了29个阳性片段,为后续的分离克隆蜡梅中被片、内被片颜色差异表达的基因奠定了基础.
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| 关 键 词： | 蜡梅 mRNA差异显示技术 差异表达 |
| 文章编号： | 0439-8114（2008）01-0008-04 |
| 收稿时间： | 2007-09-21 |
| 修稿时间： | 2007年9月21日 |
Establishment of Optimum DDRT-PCR System for Tepals of Chimonanthus praecox |
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| ZHOU Ming-qin,WANG Bo,WEN Lu,CHEN Long-qing.Establishment of Optimum DDRT-PCR System for Tepals of Chimonanthus praecox[J].Hubei Agricultural Sciences,2008,47(1):8-11. |
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| Authors: | ZHOU Ming-qin WANG Bo WEN Lu CHEN Long-qing |
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| Institution: | ZHOU Ming-qin,WANG Bo,WEN Lu,CHEN Long-qing(Key Laboratory of Horticultural Plant Biology,Ministry of Education /College of Horticulture , Forestry Science,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | In this study,mRNA differential display RT-PCR(DDRT-PCR) was first applied to study the differentially expressed genes from tepals of Chimonanthus praecox.The middle and inner tepals of the plant used in this study showed distinctly different color.Based on screening several key reaction conditions,an optimal reaction system was established and a total of 29 differentially displayed fragments were obtained.Those fragments would be very helpful for further study on cloning the genes which differentially expr... |
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| Keywords: | Chimonanthus praecox DDRT-PCR differential expression |
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