| 非洲猪瘟病毒K205R蛋白基因原核表达的研究 |
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| 引用本文： | 龚振华,莫正芬,李葳,李玉清,刘开成,史红,刘国庆,王君玮,蒋正军,王志亮.非洲猪瘟病毒K205R蛋白基因原核表达的研究[J].中国动物检疫,2010,27(12):35-36. |
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| 作者姓名： | 龚振华 莫正芬 李葳 李玉清 刘开成 史红 刘国庆 王君玮 蒋正军 王志亮 |
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| 作者单位： | 中国动物卫生与流行病学中心;湖南桃江武潭镇中心 |
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| 摘 要： | 本研究合成非洲猪瘟的K205R基因,将K205R基因克隆至pET-30c(+)质粒,构建重组原核表达质粒pET30C-K205R。该重组质粒的阅读框架正确,表达的融合蛋白的N端和C端均带6×组氨酸。pET30C-K205R-BL21菌株经培养和诱导表达后,可生产K205R可溶性蛋白,K205R可溶性蛋白可用Ni柱纯化。纯化表达K205R蛋白的ELISA试验证明,表达的K205R蛋白与猪阳性非洲猪瘟血清具有较好的特异性反应。
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| 关 键 词： | 非洲猪瘟病毒 K205R蛋白 原核表达 |
Prokaryotic Expression of K205R Gone of African Swine Fever Virus |
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| Gong Zhenhu,Me Zhengfen,Li Wei,Li Yuqing,Liu Kaicheng,Shi Hang,Liu Guoqing,Wang Junwei,Jiang Zhengjun,Wang Zhiliang.Prokaryotic Expression of K205R Gone of African Swine Fever Virus[J].China Journal Of Animal Quarantine,2010,27(12):35-36. |
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| Authors: | Gong Zhenhu Me Zhengfen Li Wei Li Yuqing Liu Kaicheng Shi Hang Liu Guoqing Wang Junwei Jiang Zhengjun Wang Zhiliang |
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| Institution: | 1(1.China Animal Health and Epidemiology Center,Qingdao,Shandong, 266032 China;2.Hunan Taojiang Wutan Middle School,413407 China) |
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| Abstract: | K205R gene of AFSV was synthesized and cloned to the plasmid pET30C to construct the recombinant prokaryotic expressed plasmid pET30C-K205R with correct reading frame and with 6×histamine both on N and C terminals of the expressed fusion protein.The recombinant plasmid pET30C-K205R was then transformed into E.coli DH5α and then into BL21 and the pET30C-K205R-BL21 strain was obtained and expressed by induction with IPTG,resulting in soluable protein K205R.ELISA with the purified K205R protein showed that the expressed K205R protein demonstrated very good specific reaction with positive ASFV serum. |
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| Keywords: | African swine fever virus K205R protein Prokaryotic expression |
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