| 杨树因伤诱导型启动子的克隆及功能分析 |
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| 引用本文： | 李强.杨树因伤诱导型启动子的克隆及功能分析[J].林业科学,1999,35(4):111-19. |
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| 作者姓名： | 李强 |
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| 作者单位： | 中国林业科学研究院林业研究所，中国科学院微生物研究所 |
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| 摘 要： | 机械损伤或病虫害侵扰引起植物一系列防御相关基因的激活，其中一些是因伤诱导表达的．Ｂｒａｄｓｈａｗ等１９８９年报道了杨树Ｗｉｎ３基因家族中的一员，其编码产物类似于白薯和豆类胰蛋白酶抑制剂，并且是因伤诱导表达的。为了更全面的了解这一基因启动子在因伤介导表达中的作用，探讨其在基因工程用于控制外源基因表达的可能性，本文分别从欧洲黑杨（Ｐ．ｎｉｇｒａ）和美洲黑杨（Ｐ．ｄｅｌｔｏｉｄｅｓ）中扩增出ｗｉｎ３基因启动子ＷＩＮＰ（７０５ｂｐ）和ＷＩＤＰ（７９１ｂｐ）．这两个启动子与来自大肠杆菌的ＧＵＳ基因融合，通过根癌农杆菌Ｔｉ质粒转化系统引入烟草中．证实了ＷＩＮＰ和ＷＩＤＰ控制的ＧＵＳ基因的表达不仅在损伤部位，而且具有系统的因伤诱导效应．对８株转基因烟草的组织化学分析表明，其表达模式与以前的报道一致。无论是在受损伤组织还是完整组织中，ＷＩＮＰ的伤诱导活性总比ＷＩＤＰ高。这两个启动子需要进一步改造以增强其活性。
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| 关 键 词： | 因伤诱导型启动子，Gus报告基因，转基因植物 |
CLONING AND CHARACTERIZATION OF WOUNDINDUCIBLE PROMOTERS FROM POPULUS |
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| Qiang Li,MengZhu Lu,YingChuan Tian,Ying Chen,YiFan Han Research
Institute of Forestry,the Chinese Academy of Forestry,Beijing,P.R. China Institute of
Microbiology,the Chinese Academy of Sciences,Beijing,P.R. China.CLONING AND CHARACTERIZATION OF WOUNDINDUCIBLE PROMOTERS FROM POPULUS[J].Scientia Silvae Sinicae,1999,35(4):111-19. |
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| Authors: | Qiang Li MengZhu Lu YingChuan Tian Ying Chen YiFan Han Research
Institute of Forestry the Chinese Academy of Forestry Beijing PR China Institute of
Microbiology the Chinese Academy of Sciences Beijing PR China |
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| Institution: | Qiang Li1*,MengZhu Lu1,YingChuan Tian2,Ying Chen1,YiFan Han1 1 Research
Institute of Forestry,the Chinese Academy of Forestry,Beijing,100091,P.R. China2 Institute of
Microbiology,the Chinese Academy of Sciences,Beijing,100080,P.R. China |
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| Abstract: | Mechanical injury or pathogen and insect invasion usually involves a series of defencerelated
gene expression in plants, some of these genes show distinct woundinducibility. One of the
woundinducible win3 multigene families from hybrid poplar, encoding a protein similar to sweet
potato sporamins and legume trypsin inhibitors, was previously isolated by Bradshaw et
al.(1989). To further understand the role of its upstream regulatory region in woundmediated
response, and its possibility to be used in genetic engineering for the control of the expression
of foreign genes, the win3 gene promoter WINP(705 bp) and WIDP(791bp)were amplified from P.
nigra and P.deltoides, respectively. Both promoters were fused to the E.coli
glucuronidase(GUS)reporter gene, and introduced into tobacco plants by virtue of
AgrobacteriumTi system. This study demonstrated that GUS expression responded to wounding
locally and remotely in transgenic tobacco under the WIN(D)P control, and the biochemical and
histochemical assays of GUS activity for 8 transgenic lines gave the similar expression pattern
as reported by Hollick et al.(1995). It was also observed that the responsibility to wounding of
WINP was always stronger than that of WIDP, both on the injured or uninjured sites. Further
modification of both promoters is necessary to increase their ability to enhance the expression
thus to be used in genetic engineering to obtain woundinducible expression of foreign gene(s). |
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| Keywords: | Woundinducible promoter GUS reporter gene Transgenic plant |
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