| 小麦成熟胚高频再生体系的建立 |
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| 引用本文： | 林国梁,王兴珍,孟亚雄,李葆春,马小乐,王化俊.小麦成熟胚高频再生体系的建立[J].西北农业学报,2012,21(10):49-52. |
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| 作者姓名： | 林国梁 王兴珍 孟亚雄 李葆春 马小乐 王化俊 |
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| 作者单位： | 甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学重点实验室;甘肃农业大学农学院;甘肃农业大学生命科学技术学院 |
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| 基金项目： | 国家支撑计划项目(2007BAD52B08);国家自然基金项目(30771331);大麦青稞产业技术体系岗位(CARS-XX);科技人员服务企业行动项目(SQ2009GJC-1005175) |
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| 摘 要： | 以甘春24成熟胚为外植体,研究消毒方式、浸种时间、接种方式、2,4-D质量浓度等处理对愈伤组织诱导与分化的影响,旨在建立一套有效的小麦高频再生体系。结果表明,不同消毒处理、2,4-D质量浓度和接种方法对成熟胚愈伤组织分化有极显著影响,不同浸种时间对成熟胚愈伤组织分化的影响差异不显著。采用25%NaClO初次消毒25min,浸种后用25%NaClO消毒10min,对愈伤组织的诱导较好,对成熟胚伤害小,且污染率低;浸种15～21h,对成熟胚愈伤组织的诱导无明显差异,但浸种18或21h有利于胚性愈伤组织的分化;成熟胚刮碎处理,有利于愈伤组织的诱导与分化,其诱导率与绿点率分别为98.33%,62.04%;当2,4-D质量浓度为2.5mg/L时,甘春24胚性愈伤组织品质较好。
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| 关 键 词： | 小麦 成熟胚 愈伤组织 |
High Frequency Plant Regeneration from Mature Embryo of Wheat |
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| LIN Guoliang,WANG Xingzhen,MENG Yaxiong,LI Baochun,MA Xiaole and WANG Huajun.High Frequency Plant Regeneration from Mature Embryo of Wheat[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(10):49-52. |
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| Authors: | LIN Guoliang WANG Xingzhen MENG Yaxiong LI Baochun MA Xiaole and WANG Huajun |
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| Institution: | 1,2(1.Gansu Key Lab of Crop Improvement & Germplasm Enhancement,Gansu Provincial Key Lab of Arid Land Crop Science, Lanzhou 730070,China;2.College of Agronomy,Gansu Agriculture University,Lanzhou 730070,China;3.College of Life Sciences and Technology of Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | Aiming to setting up an effective wheat high frequency regeneration system, the mature embryo of wheat variety Ganchun 24 was used as exophyte to explore the changes of callus induced and the influence of differentiation during the treatments of different disinfection way, including different soaking seeds time, different means of inoculation, and different 2, 4-D concentration. The results showed that different disinfection way, 2,4-D concentration and different means of inoculation had significant effects on differentiation of wheat callus of mature embryos; but different soaking seeds time had no significant effects. Using the 25% NaClO 25 min on the first disinfection, after soaking seeds utilizing 25% NaClO 10 min, the method could have good effects on callus induced and had less damage on the condition of lower rate of infection; soaking seeds of 15-21 h had no significant effects on the callus induced of wheat mature embryos, but 18 h or 21 h was better to the quality of callus induced; scraping the mature embryos was helpful for callus induction and differentiation, the rate of induction and differentiation were 98.33% and 62.04% respectively. When the 2,4-D concentration was 2.5 mg/L, the quality of embryonic callus was better of Ganchun 24. |
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| Keywords: | Wheat Mature embryo Callus |
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