| 禽副黏病毒4型荧光RT-PCR检测方法的建立 |
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| 引用本文： | 王静静,刘华雷,王英丽,郑东霞,赵云玲,吕艳,左媛媛,于松梅,王志亮.禽副黏病毒4型荧光RT-PCR检测方法的建立[J].中国动物检疫,2014,31(3):68-71. |
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| 作者姓名： | 王静静 刘华雷 王英丽 郑东霞 赵云玲 吕艳 左媛媛 于松梅 王志亮 |
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| 作者单位： | 中国动物卫生与流行病学中心,山东青岛266032 |
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| 基金项目： | 公益性行业（农业）科研专项（201303033） |
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| 摘 要： | 本研究根据GenBank发表的禽副黏病毒4型（APMV-4）基因组序列，在保守区域分别设计了特异性RT-PCR引物和荧光探针，初步建立了可用于检测APMV-4的荧光RT-PCR方法。特异性试验表明本方法特异性强，与新城疫病毒（NDV）、禽流感病毒（AIV）、传染性支气管炎病毒（IBV）、减蛋综合征病毒（EDSV）等没有交叉反应。灵敏度试验表明本方法的检测下限为1EID50，比常规RT-PCR的敏感度高100倍左右。本研究所建立的荧光RT-PCR方法具有快速、准确、灵敏等优点，可用于APMV-4样品的检测。
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| 关 键 词： | 禽副粘病毒4型 荧光RT-PCR 特异性 灵敏度 |
Establishment of Real-time RT-PCR Assayfor Detection of Avian Paramyxovirus Serotype 4 |
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| Wang Jingjing,Liu Hualei,Wang Yingli,Zheng Dongxi,Zhao Yunling,Lv Yan,Zuo Yuanyuan,Yu Songmei,Wang Zhiliang.Establishment of Real-time RT-PCR Assayfor Detection of Avian Paramyxovirus Serotype 4[J].China Journal Of Animal Quarantine,2014,31(3):68-71. |
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| Authors: | Wang Jingjing Liu Hualei Wang Yingli Zheng Dongxi Zhao Yunling Lv Yan Zuo Yuanyuan Yu Songmei Wang Zhiliang |
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| Institution: | (China Animal Health and Epidemiology Center, Qingdao, Shandong 266032) |
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| Abstract: | A real-time RT-PCR assay for detection of avian paramyxovirus serotype 4 （APMV-4） was established in this study. The specific primers and probe used in the study were designed based on the conserved region ofAPMV-4 ge- nome. The specificity of real-time RT-PCR was demonstrated by detecting five different viruses including Newcastle dis- ease virus （NDV）, avian influelaza virus （AIV）, infectious bronchitis virus （IBV）, egg drop syndrome virus （EDSV） and APMV-4. The minimal detection threshold was 1 EID50, 100 times higher than conventional RT-PCR. These results indicated that the real-time RT-PCR would be an effective assay for the rapid detection of APMV-4. |
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| Keywords: | avian paramyxovirus serotype 4 real-time RT-PCR： specificity sensitivity |
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