| 基于卧式厌氧装置的稻秸高固态消化与甲烷菌变化研究 |
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| 引用本文： | 邓玉营,阮文权,黄振兴,缪恒峰,赵明星,施万胜.基于卧式厌氧装置的稻秸高固态消化与甲烷菌变化研究[J].农业机械学报,2017,48(7):272-279. |
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| 作者姓名： | 邓玉营 阮文权 黄振兴 缪恒峰 赵明星 施万胜 |
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| 作者单位： | 江南大学;常州工程职业技术学院,江南大学,江南大学,江南大学,江南大学,江南大学 |
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| 基金项目： | 国家自然科学基金项目（51678279、21506076）、“十二五”国家科技支撑计划项目（2014BAC25B01）和常州工程职业技术学院校级科研基金项目（11130100116022） |
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| 摘 要： | 针对单一稻秸高固态厌氧消化长期运行不稳的问题,接种瘤胃内含物和厌氧污泥,在卧式反应装置中研究3个有机负荷(OLR)下消化特性。结果表明,体系最高容积产气率达到了1.04 L/(L·d)。当OLR为2.26 g/(L·d)时,甲烷体积分数均值为54.39%,甲烷产率为280.90 m L/g,达到了稻秸理论产值的80.29%。卧式装置中半纤维素和纤维素最高降解率分别达到了49.71%和31.25%;纤维素酶活性显著提高,有利于纤维素的降解。当OLR升高到2.47 g/(L·d)时,氨氮质量浓度均值达到了1 082.63 mg/L。固体样品中嗜氢型Methanobacteriales数量从1.70×10~9拷贝数/g下降至1.04×10~6拷贝数/g;而嗜乙酸型Methanosarcinales数量从7.89×10~6拷贝数/g增加至9.44×10~6拷贝数/g,甲烷产率下降为256.54 m L/g。此时厌氧装置中丙酸质量浓度均值达到了253.32 mg/L。从而明确了稻秸高固态体系中产甲烷菌结构的变化。
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| 关 键 词： | 卧式反应装置 稻秸 高固态消化 甲烷菌群 |
| 收稿时间： | 2017/5/16 0:00:00 |
High Solid-state Digestion of Rice Straw and Changes of Methanogens in Horizontal Anaerobic Reactor |
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| DENG Yuying,RUAN Wenquan,HUANG Zhenxing,MIAO Hengfeng,ZHAO Mingxing and SHI Wansheng.High Solid-state Digestion of Rice Straw and Changes of Methanogens in Horizontal Anaerobic Reactor[J].Transactions of the Chinese Society of Agricultural Machinery,2017,48(7):272-279. |
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| Authors: | DENG Yuying RUAN Wenquan HUANG Zhenxing MIAO Hengfeng ZHAO Mingxing and SHI Wansheng |
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| Institution: | Jiangnan University;Changzhou Vocational Institute of Engineering,Jiangnan University,Jiangnan University,Jiangnan University,Jiangnan University and Jiangnan University |
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| Abstract: | Instability of high solid-state anaerobic digestion often happened during the long term operation of reactor. To investigate underlying methanism of this problem, the characteristics of semi continuous anaerobic digestion for rice straw were studied with three organic loading rates (OLR) in a horizontal reactor, when being co-inoculated with ruminal contents and anaerobic sludge. The results indicated that the highest volume biogas production reached 1.04L/(L·d) in the reactor. The average CH4 productivity reached 280.90mL/g VS with 54.39% CH4 volume content at the OLR of 2.26g/(L·d), which was up to 80.29% of the theoretical value of rice straw. The highest digestion rates of hemicellulose and cellulose were 49.71% and 31.25%, respectively. And the cellulase activities were increased obviously in the horizontal reactor, which was indispensable to the degradation of cellulose. At the OLR of 2.47g/(L·d), the average concentration of ammonium nitrogen reached 1082.63mg/L. Especially the highest concentration reached 1196.37mg/L after 192d of operation. It was indicated that the methanogens suffered different influences from ammonium nitrogen using quantitative real time PCR(Q-PCR). Specifically, the hydrogenotrophic Methanobacteriales was decreased to 1.04×106 copies/g from 1.70×109copies/g dominated in the solid residues and CH4 productivity was decreased to 256.54mL/g for being inhibited significantly. In contrast, the acetotrophic Methanosarcinales was increased to 9.44×106 copies/g from 7.89×106 copies/g. At the same time, the average propionate concentration reached 253.32mg/L in the anaerobic reactor. Thus the study revealed the adaptive shifts of underlying methanogenic communities by ammonium nitrogen inhibition during the high solid state digestion of rice straw. |
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| Keywords: | horizontal reactor rice straw high solid-state digestion methanogenic community |
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