| 乌贼墨中多酚氧化酶的分离及纯化 |
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| 引用本文： | 郑校先,戚晓玉,等.乌贼墨中多酚氧化酶的分离及纯化[J].上海水产大学学报,2001,10(2):154-157. |
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| 作者姓名： | 郑校先 戚晓玉 |
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| 作者单位： | 郑校先（上海水产大学食品学院,上海 200090）
戚晓玉（上海水产大学食品学院,上海 200090）
周培根（上海水产大学食品学院,上海 200090）
江津津（上海水产大学食品学院,上海 200090） |
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| 摘 要： | 用pH7.2磷酸提取缓冲液从乌贼墨汁澡提取多酚氧化酶，提取的粗酶液经30%-80%饱和度硫酸铵分级沉淀、透析、DEAE-SepharoseCL-6B柱层析和SephadexG-150柱层析后，得到纯化的多酚氧化酶，纯化们数为10.78，纯化后的酶液经聚丙烯酰胺凝胶电泳，分别采用银染色和多酚氧化酶活性染色均显示一条电泳带。
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| 关 键 词： | 乌贼墨 多酚氧化酶 纯化 |
| 文章编号： | 1004-7271(2001)02-0154-04 |
| 修稿时间： | 2001年3月14日 |
Purification of polyphenol oxidase from cuttlefish ink |
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| ZHENG Xiao xian,QI Xiao yu,ZHOU Pei gen,JIANG Jin jin.Purification of polyphenol oxidase from cuttlefish ink[J].Journal of Shanghai Fisheries University,2001,10(2):154-157. |
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| Authors: | ZHENG Xiao xian QI Xiao yu ZHOU Pei gen JIANG Jin jin |
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| Abstract: | Polyphenol oxidase (PPO) was extracted from the cuttlefish ink with pH 7.2 sodium phosphate extraction buffer. The crude enzyme extract was fractionated with solid ammonium sulfate of 30%-80% saturation. After dialysis, the enzyme solution was purified by DEAE Sepharose CL 6B column chromatography and Sephadex G 150 column chromatography respectively. A 10.78 fold purification of PPO was obtained. The result from polyacrylamide gel electrophoresis of purified enzyme indicated that only one band was found using silver stain and PPO activity stain, respectively. |
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| Keywords: | cuttlefish ink Polyphenol oxidase purification |
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