| 丹顶鹤、灰鹤RAPD图谱的比较 |
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| 引用本文： | 李拥军,赵万里,丁家桐,陈浩,王会,吕士成.丹顶鹤、灰鹤RAPD图谱的比较[J].扬州大学学报(农业与生命科学版),1999(4). |
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| 作者姓名： | 李拥军 赵万里 丁家桐 陈浩 王会 吕士成 |
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| 作者单位： | 扬州大学畜牧兽医学院动物科学系!扬州225009(李拥军，赵万里，丁家桐)，盐城国家级珍禽自然保护区!盐城224300(陈浩，王会，吕士成) |
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| 基金项目： | 江苏省应用基础资助项目 |
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| 摘 要： | 通过筛选的 1 0个随机引物 ,利用 RAPD技术 ,对丹顶鹤及灰鹤的池 DN A进行了多态性研究。结果表明 :1 0个引物共产生条带 1 3 4个 ,扩增产物片段长度一般为 3 1 0 bp～ 2 .1 kb;利用的引物不同 ,鹤种间的特异性条带也不同 ,从而可以分析鹤种间的遗传差异。根据池 DN A多态性分析 ,两鹤种间的相似性指数为 0 .2 2 3。说明 RAPD技术可作为鹤类遗传结构分析的辅助手段之一
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| 关 键 词： | RAPD技术 丹顶鹤 灰鹤 池DNA 遗传结构 相似性指数 |
COMPARISON OF RAPD FIGERPRINT BETWEEN RED CROWNED AND COMMON CRANES |
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| LI Yong jun,ZHAO Wan li,DING Jia tong,CHEN Hao,WANG Hui,LU Shi cheng.COMPARISON OF RAPD FIGERPRINT BETWEEN RED CROWNED AND COMMON CRANES[J].Journal of Yangzhou University:Agricultural and Life Science Edition,1999(4). |
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| Authors: | LI Yong jun ZHAO Wan li DING Jia tong CHEN Hao WANG Hui LU Shi cheng |
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| Institution: | LI Yong jun 1 ZHAO Wan li 1 DING Jia tong 1 CHEN Hao 2 WANG Hui 2 LU Shi cheng 2 |
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| Abstract: | Genetic constitution of population in red crowned cranes and common cranes were analysed by RAPD technique, and 10 selected random primers were used to study DNA polymorphism of pooled DNA in the 2 species. The results indicated that 134 bands were brought about by 10 random primers, and fragment lengths of amplified products were generally from 310 bp to 2.1 kb. Every species has specific bands respectively because of different primers, which may research genetic difference among species. The similar index between Red-crowned and Common cranes according to pooled DNA was 0.223. In conclusion, it was feasible and efficient that RAPD technique was used as assistant means in analysing genetic constitution of population. |
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| Keywords: | RAPD technique red crowned crane common crane pooled DNA genetic constitution similar index |
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