Cloning and Activity Analysis of Promoter of RanGTPase Gene from Shrimp,Marsupenaeus japonicus |
| |
| Authors: | Fang Han Zhi‐Yong Wang |
| |
| Institution: | 1. Key Laboratory of Healthy Mariculture in the East China Sea, Ministry of Agriculture;2. Fisheries college, Jimei University, , Xiamen, 361021, China |
| |
| Abstract: | RanGTPases, one superfamily of small G protein family, have been demonstrated to be involved in the innate immune system and they are up‐regulated after pathogen infection. However, the regulation mechanism of RanGTPases remains unclear. In this study, the 5′ flanking region of shrimp RanGTPase gene was cloned and sequenced. To detect the activity of the promoter, pIZΔIE/EGFP was modified from pIZ/V5‐His by replacing the OPIE2 promoter with this promoter and inserting enhanced green fluorescence protein (EGFP) gene downstream. The promoter can drive the expression of reporter EGFP gene in Hi5 insect cells, indicating the promoter has activity. These results extend our previous findings and provide insights into the molecular regulation of RanGTPase gene expression, which will be helpful for shrimp viral disease control. |
| |
| Keywords: | |
|
|
