Heat Shock Protein Regulation in Rainbow Trout,Oncorhynchus mykiss,is Altered by Dietary Soybean Meal Inclusion and Anti‐Phopholipase A2 Antibody |
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| Authors: | Frederic T Barrows Charles E Smith Jurij M Wacyk Madison S Powell Ronald W Hardy Eric A Shelden |
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| Institution: | 1. USDA‐ARS, Trout Grains Project, Hagerman Fish Culture Experiment Station, 3059F National Fish Hatchery Road, Hagerman, Idaho 83332, USA;2. Aquaculture & Pathology Consulting, 717 SE 5th Street, College Place, Washington 99324, USA;3. Hagerman Fish Culture Experimental Station and Department of Animal & Veterinary Sciences, University of Idaho, 3059F National Fish Hatchery Road, Hagerman, Idaho 83332, USA;4. Washington State University, School of Molecular Biosciences, PO Box 647521, Pullman, WA 99164‐4234, USA |
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| Abstract: | Heat shock proteins (Hsps) can promote integrity of damaged intestinal epithelial tissues. The aim of this study was to determine the effects of a commercially available anti‐phospholipase A2 antibody on growth, feed efficiency, Hsp expression, and intestinal health of rainbow trout fed diets containing high levels of soybean meal (SBM). Six practical‐type diets (45% protein; 15% fat) were formulated to contain 0, 17.5, or 35% SBM with and without antibody. Each diet was fed to three replicate tanks of fish (20 fish/tank; initial weight 21 g) for 9 wk. RNA was isolated from liver and intestinal tissues (n = 3/tank) to detect alterations in Hsp 27, Hsp 70, and Hsp 90 expression. Fish fed a diet containing 35% SBM had decreased growth and intestinal damage. Hsp 27 and Hsp 90 mRNA was elevated in fish fed 35% SBM. Fish fed 35% SBM with antibody had lower Hsp 90 mRNA expression than fish without antibody. These data indicate that feeding rainbow trout high levels of SBM alters the expression of some Hsps and anti‐phospholipase A2 antibody supplementation does not normalize growth or prevent distal enteritis in fish fed 35% SBM. |
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