Mapping powdery mildew resistance gene in V97‐3000 soybean |
| |
| Authors: | Yueqiang Wang Ainong Shi Bo Zhang Pengyin Chen |
| |
| Institution: | 1. Jilin Academy of Agricultural Sciences, , Changchun, 130033 China;2. Horticulture Department, University of Arkansas, , Fayetteville, AR, 72701 USA;3. Virginia State University, , Petersburg, VA, 23112 USA;4. Department of Crop, Soil, and Environmental Sciences, University of Arkansas, , Fayetteville, AR, 72701 USA |
| |
| Abstract: | V97‐3000 is a maturity group (MG) V soybean breeding line derived from SS 516 × V90‐2592 (Vance × V81‐1325) with high stachyose, small seed and powdery mildew resistance. A total of 53 F2:3 families were derived from a cross between V97‐3000 and a powdery mildew susceptible line V99‐5089. The 53 F2:3 families, each with 30 plants, were grown in the greenhouse for powdery mildew evaluation, and the corresponding 53 F2 plants were genotyped using simple sequence repeat (SSR) markers. Results showed that the 53 F2:3 families segregated in ratio of one resistant : two segregating : one susceptible (13 : 26 : 14) and the 26 segregating F2:3 families each exhibited a good fit to three resistant : one susceptible, indicating that resistance to powdery mildew is conditioned by a single dominant gene. The gene for powdery mildew resistance in V97‐3000 was mapped on chromosome 16 linkage group (LG) J] flanked by Satt547 and Sat_396 on one side and Sat_393 on the other side with 3.8 cM and 3.9 cM distance, respectively. This study provides a new source of powdery mildew resistance and information of genetic location of the resistance gene and linked markers, which is useful for breeders selecting powdery mildew resistance through marker‐assisted selection (MAS) in soybean breeding programmes. |
| |
| Keywords: | soybean (Glycine max) powdery mildew resistance |
|
|
