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Evaluation of genetic markers for prediction of preharvest sprouting tolerance in hard white winter wheats
Authors:Robert A Graybosch  Paul St Amand  Guihua Bai
Institution:1. USDA‐ARS, 137 Keim, University of Nebraska, , Lincoln, NE, 68583 USA;2. USDA‐ARS‐HWWGRU 4008 Throckmorton Hall, Kansas State University, , Manhattan, KS, 66506 USA
Abstract:Preharvest sprouting of wheat results in significant financial loss at all steps in the production and marketing chain. Due to its intermittent nature in many wheat‐growing regions, direct selection for tolerance to preharvest sprouting is difficult. DNA markers linked to genes conditioning tolerance offer a more consistent and reliable approach to genetic improvement in tolerance. This investigation assessed the value of previously identified markers linked to quantitative trait loci contributing to tolerance, across multiple genetic backgrounds. A significant contribution to tolerance was demonstrated for QPhs.pseru‐3AS, previously identified from the hard white winter wheat ‘Rio Blanco’. Marker alleles for this locus were associated with enhanced tolerance in three of four investigated populations. In addition, positive contributions of QPhs.pseru‐2B1 and QPhs.pseru‐2B2 also were documented. Simultaneous selection for putative marker alleles at two independent loci resulted in significantly higher mean tolerance scores. DNA markers linked to loci contributing to variation in preharvest sprouting tolerance offer an efficient and effective alternative to direct phenotypic selection.
Keywords:wheat  pre harvest sprouting  DNA markers  chromosome 3AS    Rio Blanco’  
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