| 柑桔木质陷孔病类病毒贵州分离物的分子鉴定 |
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| 引用本文： | 王雪峰,唐科志,李中安,周彦,兰建强,周常勇.柑桔木质陷孔病类病毒贵州分离物的分子鉴定[J].云南农业大学学报,2009,24(2):181-184. |
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| 作者姓名： | 王雪峰 唐科志 李中安 周彦 兰建强 周常勇 |
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| 作者单位： | 王雪峰,WANG Xue-feng(云南农业大学,植物保护学院,云南,昆明,650201;西南大学,柑桔研究所,重庆,北碚,400712);唐科志,李中安,周彦,周常勇,TANG Ke-zhi,LI Zhong-an,ZHOU Yan,ZHOU Chang-yong(西南大学,柑桔研究所,重庆,北碚,400712);兰建强,LAN Jian-qiang(云南农业大学,植物保护学院,云南,昆明,650201)
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| 摘 要： | 从采自贵州感染了啤酒花矮化类病毒（Hop stunt viroid, HSVd）的柑桔植株中抽提总核酸，通过一对鉴别引物，经RT-PCR扩增初步筛选出柑桔木质陷孔病类病毒（Citrus cachexia viroid, CCaVd）。扩增该类病毒的全长核苷酸片段，通过克隆和测序，结果发现，该片段全长298bp，与GenBank中报道的木质陷孔病类病毒分离物同源性达到96％以上。这是中国发生的木质陷孔病类病毒的分子生物学特征的首次报道。
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| 关 键 词： | 柑桔木质陷孔病类病毒 啤酒花矮化类病毒 RT-PCR 分子生物学特征 |
| 收稿时间： | 2008-4-24 |
| 修稿时间： | 2008-9-2 |
Molecular Identification of Guizhou Isolate of Citrus Cachexia Viroid |
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| WANG Xue-feng,TANG Ke-zhi,LI Zhong-an,ZHOU Yan,LAN Jian-qiang,ZHOU Chang-yong.Molecular Identification of Guizhou Isolate of Citrus Cachexia Viroid[J].Journal of Yunnan Agricultural University,2009,24(2):181-184. |
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| Authors: | WANG Xue-feng TANG Ke-zhi LI Zhong-an ZHOU Yan LAN Jian-qiang ZHOU Chang-yong |
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| Institution: | 1College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;2Citrus Research Institute, Southwest University, Chongqing 400712, China |
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| Abstract: | Citrus cachexia viroid (CCaVd) was detected from hop stunt viroid(HSVd) infected citrus collected from Gouzhou by RT-PCR with a discrimination primer pair. The full length fragment of the CCaVd isolate was amplified by a pair of specific primer. Cloning and sequencing of the fragment revealed it was 298 bp long with above 96% identity compared with reported sequences of CCaVd in GenBank. This is the first report on the molecular characteristics of CCaVd in China. |
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| Keywords: | RT-PCR |
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