| 龙胆草组织培养及其无性系的研究 |
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| 引用本文： | 文伟,杨骥.龙胆草组织培养及其无性系的研究[J].安徽农业科学,2010,38(12):6131-6133. |
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| 作者姓名： | 文伟 杨骥 |
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| 作者单位： | 辽宁铁岭师范高等专科学校,辽宁铁岭,112001;辽宁铁岭师范高等专科学校,辽宁铁岭,112001 |
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| 摘 要： | 目的]建立龙胆草组织苗繁育体系。方法]以龙胆草的嫩茎为材料,进行愈伤组织的诱导与分化、不定芽的分化与生根、试管苗移栽与移植的研究。结果]MS＋0.2mg/LBA＋1.0～1.5mg/L2,4-D是诱导嫩茎形成具有分化能力愈伤组织的理想培养基;MS＋1.2mg/LAgNO3＋0.6mg/LBA＋0.1mg/LNAA是愈伤组织和不定芽分化培养的理想培养基;1/2MS＋0.1mg/LIAA＋0.3mg/LNAA是龙胆草试管苗生根培养和生根继代培养的理想培养基;移植到山坡上的试管苗保持了龙胆草的各项植物学性状。结论]在该试验条件下,培育的试管苗移栽成活率达96%,说明此方法完全可以用于大田生产。
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| 关 键 词： | 龙胆草 组织培养 无性系 |
Study on the Tissue Culture and Propagation System of Gentiana scabra Bnnge |
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| Institution: | WEN Wei et al(Tieling Normal College of Liaoning Province,Tieling,Liaoning 112001) |
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| Abstract: | Objective] The research aimed to establish the propagation system of tissue culture seedlings of Gentiana scabra Bnnge.Method] Using the tender stems of G.scabra as materials,the callus induction and differentiation,the differentiation and rooting of adventitious buds,the transplanting of tube seedlings were studied.Result] The ideal medium for inducing the tender stems to form the differentiation calli was MS+0.2 mg/L BA+1.0-1.5 mg/L 2,4-D.The ideal medium for the differentiation culture of calli and adventitious buds was MS+1.2 mg/L AgNO3+0.6 mg/L BA+0.1 mg/L NAA.The ideal medium for the rooting culture and rooting sub-culture of G.scabra tube seedlings was 1/2 MS+0.1 mg/L IAA+0.3 mg/L NAA.The botany characters of G.scabra tube seedlings transplanted on the hillside.Conclusion] Under these experimental conditions,the transplanting survival ratio of cultured tube seedlings reached 96%,indicating that the method can be used in field production completely. |
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| Keywords: | Gentiana scabra Bnnge Tissue culture Clone |
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