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| 菜薹抽薹性状的ISSR和SRAP分析 | |
| 引用本文： | 冒维维,高红胜,薄天岳,马金骏,徐东进,陈学好,贾志明,王永莉.菜薹抽薹性状的ISSR和SRAP分析[J].江苏农业学报,2009,25(4). |
| 作者姓名： | 冒维维 高红胜 薄天岳 马金骏 徐东进 陈学好 贾志明 王永莉 |
| 作者单位： | 1. 扬州大学园艺与植物保护学院,江苏,扬州,225009;江苏省姜堰市农业局,江苏,泰州,225529 2. 扬州大学园艺与植物保护学院,江苏,扬州,225009 3. 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106 4. 扬州帮达蔬菜研究所,江苏,扬州,225009 |
| 基金项目： | 江苏省农业高技术项目 |
| 摘 要： | 以早抽薹品种CT07和晚抽薹品种T0601为亲本构建F2分离群体,采用分离集团混合分析法 (Bulked segregant analysis,BSA)筛选与菜薹抽薹基因连锁的分子标记.用45条ISSR(Inter-simple sequence repeat,简单重复序列间扩增)引物和20对SRAP(Sequence related amplified polymorphism,序列相关扩增多态性)引物组合进行PCR扩增筛选,其中UBC834、UBC876、E13M4和E5M7对亲本和DNA池的扩增图谱表明,4个引物的差异条带均出现在早抽薹品种CT07和早现蕾(抽薹)池中.经F2代群体单株验证,4个标记均与早抽薹基因相连锁,且标记E13M4最近,距离为16.8 cM,这为菜薹抽薹基因的定位和分子标记辅助育种奠定了基础. |
| 关 键 词： | 菜薹 抽薹性状 分离集团混合分析(BSA) |
Analysis of Bolting Trait of Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee by ISSR and SRAP Makers |
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| MAO Wei-wei,GAO Hong-sheng,BO Tian-yue,MA Jin-jun,XU Dong-jin,CHEN Xue-hao,JIA Zhi-ming,WANG Yong-li.Analysis of Bolting Trait of Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee by ISSR and SRAP Makers[J].Jiangsu Journal of Agricultural Sciences,2009,25(4). | |
| Authors: | MAO Wei-wei GAO Hong-sheng BO Tian-yue MA Jin-jun XU Dong-jin CHEN Xue-hao JIA Zhi-ming WANG Yong-li |
| Institution: | 1.College of Horticulture and Plant Protection;Yangzhou University;Yangzhou 225009;China;2.Jiangyan Agricultural Bureau;Taizhou 225529;3.Horticultural Research Institute;Shanghai Academy of Agricultural Sciences;Shanghai Key Lab of Protected Horticultural Technology;Shanghai 201106;4.Bangda Vegetable Research Institute of Yangzhou;China |
| Abstract: | The F2 population derived from a cross between CT07(early bolting) and T0601(late bolting) was used to identify bolting trait using ISSR and SRAP makers by BSA analysis.45 ISSR primers and 20 SRAP primers combinations were used to screen polymorphism in parents and DNA pools.PCR pattern in parents and DNA pools showed different fragment appeared in CT07 and early bolting pools by UBC834,UBC876,E13M4 and E5M7 primers.Bolting trait was linked to four makers and the closest marker to bolting gene was E13M4 and... |
| Keywords: | ISSR SRAP |
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