Interference with cell-to-cell movement of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis> by transient overexpression of <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> KELP homologs from different plant species |
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| Authors: | Nobumitsu Sasaki Tatsuro Odawara Masakazu Deguchi Yasuhiko Matsushita Hiroshi Nyunoya |
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| Institution: | (1) Gene Research Center, Tokyo University of Agriculture and Technology, Fuchu Tokyo, 183-8509, Japan |
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| Abstract: | The KELP protein of Arabidopsis thaliana (AtKELP), which binds to the Tomato mosaic virus (ToMV) movement protein in vitro, can interfere with cell-to-cell movement of the virus under transient overexpression conditions.
In this study, we constructed expression plasmids encoding a fluorescent protein fused to AtKELP or to its homolog from Brassica, Nicotiana, Solanum, or Oryza species. When expressed transiently, all the AtKELP homologs inhibited the cell-to-cell movement of ToMV with different efficiencies.
The results of domain swapping between two selected AtKELP homologs suggest the importance of combination between the N- and
C-terminal half regions for high inhibitory function. |
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