| 养分胁迫对籽粒灌浆期水稻叶片衰老影响的蛋白质组学分析 |
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| 引用本文： | 谢金水,邵彩虹,唐秀英,石庆华.养分胁迫对籽粒灌浆期水稻叶片衰老影响的蛋白质组学分析[J].中国水稻科学,2011,25(2):143-149. |
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| 作者姓名： | 谢金水 邵彩虹 唐秀英 石庆华 |
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| 作者单位： | 1. 江西农业大学,农学院,江西,南昌,330045
2. 江西省农业科学院,土壤肥料与资源环境研究所,江西,甫昌,330200 |
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| 基金项目： | 国家科技支撑计划资助项目(2006BAD02A04) |
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| 摘 要： | 采用蛋白质组学分析技术，对养分胁迫下杂交水稻威优916功能叶片蛋白质组差异表达进行研究，以揭示养分胁迫影响籽粒灌浆期水稻衰老的分子机理。经双向电泳分离，从功能叶中获得34个差异表达蛋白质，其中26个蛋白质功能得到鉴定，包括11个参与光合作用蛋白质，4个参与呼吸作用蛋白质，10个参与抗氧化及信号传导蛋白质和1个未知功能蛋白质。分析结果表明，养分胁迫下，灌浆期水稻叶片光合作用及呼吸作用显著下降；清除活性氧类蛋白质表达量下调，活性氧生成蛋白质及胁迫信号传导蛋白质表达量上调，水稻抗性减弱，叶片中活性氧积累，水稻衰老加剧。
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| 关 键 词： | 水稻 养分胁迫 功能叶 衰老 蛋白质组学 |
| 收稿时间： | 2010-05-07; |
Proteomics Analysis of Nutrient Stress Effect on Rice Leaf Senescence at Grain Filling Stage |
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| XIE Jin-shui,SHAO Cai-hong,TANG Xiu-ying,SHI Qing-hua.Proteomics Analysis of Nutrient Stress Effect on Rice Leaf Senescence at Grain Filling Stage[J].Chinese Journal of Rice Science,2011,25(2):143-149. |
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| Authors: | XIE Jin-shui SHAO Cai-hong TANG Xiu-ying SHI Qing-hua |
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| Institution: | XIE Jin-shui1,SHAO Cai-hong2,TANG Xiu-ying1,SHI Qing-hua1,(1College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China,2Soil and Fertilizer & Resource and Environment Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,) |
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| Abstract: | In order to reveal the molecular senescence mechanism of rice under nutrient stress at grain filling stage,the protein expression profile of functional leaves in hybrid rice Weiyou 916 was investigated by the approach of proteomics.Proteins were extracted from rice flag leaf and separated by two-dimensional gel electrophoresis.Thirty-four differentially expressed proteins were obtained,among which 26 were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry,and categori... |
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| Keywords: | rice nutrient stress functional leaf senescence proteomics |
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