Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks |
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| Authors: | Zhao Namula Yoko Sato Risa Kodama Kouta Morinaga Vien Viet Luu Masayasu Taniguchi Michiko Nakai Fuminori Tanihara Kazuhiro Kikuchi Takashi Nagai Takeshige Otoi |
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| Institution: | 1. The United Graduate School of Veterinary Science, Yamaguchi University, , Yamaguchi, Japan;2. National Institute of Agrobiological Sciences, , Tsukuba, Japan;3. National Agriculture and Food Research Organization (NARO), NARO Institute of Livestock and Grassland Science (NILGS), , Tsukuba, Japan |
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| Abstract: | This study investigated the effects of skim milk on the quality and fertility of boar spermatozoa under long‐term chilled preservation. Semen samples were stored in Modena solution supplemented with 0 (control) to 50 mg/mL skim milk at 5°C for 4 weeks; spermatozoa stored with 7.5 and 15 mg/mL of skim milk (7.5‐SM and 15‐SM groups, respectively) exhibited significantly higher motility indices than those of the control group up to 3 weeks (P < 0.05), and the 7.5‐SM group showed improved motility indices even after 4 weeks (P < 0.05). In vitro fertilization using spermatozoa in the 7.5‐SM and 15‐SM groups stored at 5°C for 2 weeks showed significantly higher fertilization rates of spermatozoa and the development rates to blastocyst than the control group (P < 0.05), and the 7.5‐SM group showed similar rates of fertilization and blastocyst formation in the fresh non‐stored spermatozoa group. After artificial insemination using spermatozoa stored for 2 weeks in the 7.5‐SM group, healthy piglets were obtained. Boar spermatozoa can be stored at 5°C in a Modena solution containing skim milk. Supplementation of 7.5 mg/mL skim milk improves boar spermatozoa motility and fertility even after liquid preservation at 5°C for 2 weeks. |
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| Keywords: | AI boar semen chilled preservation skim milk sperm quality |
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