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红螯螯虾输精管的结构及精荚的形成
引用本文:丁银娣,贾林芝,马强,赵云龙,王群.红螯螯虾输精管的结构及精荚的形成[J].水产学报,2009,33(1):78-86.
作者姓名:丁银娣  贾林芝  马强  赵云龙  王群
作者单位:华东师范大学生命科学学院,上海,200062
摘    要:运用组织学、组织化学和蛋白电泳等多种方法,对红螯螯虾输精管结构和精荚形成进行研究,结果发现,红螯螯虾精荚为一个连续的管状结构,由精子群、精荚基质和精荚壁组成; 精荚壁完全包裹精子,分初级、次级精荚壁2层,初级精荚壁由致密的纤丝组成,包裹精子和精荚基质;次级精荚壁由大小不等的小泡和疏松的纤丝组成,包裹在初级精荚壁之外。红螯螯虾输精管可分为前、中、后输精管。其上皮细胞均具有合成、分泌精荚形成物质的功能。前输精管卷曲段的高柱状上皮,分泌弱嗜酸性丝状分泌物,主要成分为中性粘多糖和蛋白质,形成均匀的初级精荚壁;中输精管的高柱状上皮,成丛状向内腔突起,具有分泌泡,其分泌物为嗜酸性、颗粒状物质,主要成分为酸性粘多糖和蛋白质,形成不均匀,黏性极好的次级精荚壁。后输精管结构较简单,肌层较前两者厚,同时管腔更大,其内精荚结构完整。后输精管与射精管相连处有一狭部,内有瓣膜相隔。射精管肌层厚,上皮明显突起,内无精荚。

关 键 词:红螯螯虾  输精管  精荚
收稿时间:2007/12/7 0:00:00
修稿时间:4/8/2008 12:00:00 AM

The Structure of Vas deferens and Formation of Spermatophore in Crayfish: Cherax quadricairnatus
DING Yin-di,JIA Lin-zhi,MA Qiang,ZHAO Yun-long,WANG Qun.The Structure of Vas deferens and Formation of Spermatophore in Crayfish: Cherax quadricairnatus[J].Journal of Fisheries of China,2009,33(1):78-86.
Authors:DING Yin-di  JIA Lin-zhi  MA Qiang  ZHAO Yun-long  WANG Qun
Institution:school of life science, east china normal university
Abstract:Crayfishs were collected from artificial ponds in Zhejiang Institute of Freshwater Fisheries. The samples were observed under microscope after being sacrificed and investigated with histological, histochemical, biochemical methods and chromatography. The C. quadricarinatus spermatophore is comprised of a highly convoluted tube and a firm membrane forming a cord-like mass, This spermatophoric ribbon is embedded in a gelatinous matrix. The structure of C. quadricarinatus spermatophore was observed under the electron microscopy, which is consisted of sperm mass, spermatophore matrix and spermatophore wall. Sperm mass is embedded in a dense fibrillar matrix and with an acellular wall surrounds both of them. In this study, the structure of the spermatophore wall in the vas deferens was also investigated using electron microscopy and biochemical test. The wall of the spermatophore is composed of two concentric layers: a thin primary spermatophore layer which is a fibrillar structure that directly surrounds the sperm mass and spermatophore matrix, and a thick secondary layer which contains heterogeneous vesicles and loose filament surrounds the primary spermatophore layer. The vas deferens of C. quadricarinatus consists of an anterior vas deferens, a middle vas deferens, and a posterior vas deferens. The histology of the inner epithelial cells of the vas deferens contributing to the spermatophore mass is described. The inner epithelial cell of the coiling anterior vas deferens secretes the weakly acidophilic filament that forms the equable primary layer of spermatophore. Neutral muopolysaccharides and protein are the main components of the primary layer. There are incrassate epithelial cells of middle vas deferens, which are fasciculate high cellula columnoepithelialis. In the middle of epithelial cells, we observed some secretory vacuoles with acidophily vesicular secretion, which formed the inequable secondary layer. Acidic muopolysaccharides and protein are the main components of the secondary layer. Spermatophores structure are complete in posterior vas deferens which has a simpler structure than the former two but incrassate muscular layer and lager tube. The posterior vas deferens links with ejaculatory duct. Between the posterior vas deferens and the ejaculatory duct, there is a bottleneck with two valves inside. The ejaculatory duct has an incrassate muscular layer and protuberant inner epithelial cells. No spermatophore in the ejaculatory duct is visible.
Keywords:Cherax quadricairnatus  vas deferens  Formation of spermatophore  
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