| 23个石榴基因型遗传多样性的SRAP分析 |
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| 引用本文： | 张四普,汪良驹,曹尚银,刘涛.23个石榴基因型遗传多样性的SRAP分析[J].果树学报,2008,25(5):655-660. |
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| 作者姓名： | 张四普 汪良驹 曹尚银 刘涛 |
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| 作者单位： | [1]南京农业大学园艺学院,南京210095 [2]河南省农业科学院园艺研究所,郑州450002 [3]中国农业科学院郑州果树研究所,郑州450009 [4]徐州市贾汪区水利局,江苏徐州221011 |
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| 摘 要： | 以23个石榴基因型为试材,利用7对SRAP引物进行PCR扩增,共扩增出1380个条带,其中多态性条带662个,多态率为48%。应用DPS分析软件构建UPGMA聚类图,23个基因型遗传距离在0.0769～0.4131。在遗传距离0.33处,可将石榴基因型分为A、B、C、D和E5个类群。4种单瓣白花基因型都聚类在A组;B组都是单瓣红花、味甜的鲜食品种;C组中鲜食品种果皮都不着色;4种不同花色或叶型的单瓣、赏食兼用基因型则聚在D组;观赏型墨石榴与其它基因型显著不同,单独聚为一类(E组)。引物对组合ME4/EM4-800bp缺失条带是青皮石榴的特异缺失标记,ME4/EM4-480bp缺失条带是峄城红和河阴软籽石榴的特异缺失标记,ME2/EM3-120bp缺失条带是峄城红、白皮酸和大青皮的特异缺失标记。研究结果表明,石榴基因型有着丰富的遗传多样性,SRAP技术可有效运用于石榴基因型的遗传分析。
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| 关 键 词： | 石榴 SRAP 遗传多样性 |
Analysis of genetic diversity of 23 pomegranate genotypes by SRAP |
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| ZHANG Si-pu,WANG Liang-ju,CAO Shang-yin,LIU Tao.Analysis of genetic diversity of 23 pomegranate genotypes by SRAP[J].Journal of Fruit Science,2008,25(5):655-660. |
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| Authors: | ZHANG Si-pu WANG Liang-ju CAO Shang-yin LIU Tao |
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| Institution: | ZHANG Si-pu1,2,WANG Liang-ju1,CAO Shang-yin3,LIU Tao4(1College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China,2Henan Horticultural Resarch Institute,Zhengzhou,Henan 45002 China,3Zhengzhou Fruit Research Institute,CAAS,Henan 450009 China,4Jiawang District Hydrological Bureau,Xuzhou,Jiangsu 221011 China) |
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| Abstract: | SRAP(Sequence Related Amplified Polymorphism)was applied to analyze 23 pomegranate genotypes(Punica spp.)by using 7 pairs of primers.About 1 380 bands were amplified,of which 662 were polymorphic,and the polymorphic percentage of bands was 48%.A dendrogram,conducted by UPGMA method using DPS software,showed that the range of genetic distance among 23 pomegranate genotypes was 0.076 9～0.413 1.The genotypes could be divided into five sub-clusters(A,B,C,D and E)at the genetic distance of 0.33.Four white single-petal flower types are all clustered into group A,while the genotypes are all single-petal flower and sweet flesh cultivars in group B.The peel of cultivars in group C was all non-red,and four ornamental and flesh types of single-petal flower with different floral color and leaf shapes were clustered into group D.One ornamental type,P.granatum var.nigra,was significantly different from other types and was clustered into group E.Genotype Qingpi could be distinguished from all the rest based only on the lack of ME4/EM4-800 bp fragment,and Yichenghong and Heyinruanzi on the lack of ME4/EM4-480 bp fragment.Yichenghong,Baipisuan and Daqingpi could be identified by the lack of ME2/EM3-120 bp fragment.These results indicated that there was plentiful genetic diversity among pomegranate genotypes and SRAP could be effectively applied to analyze genetic diversity among pomegranate genotypes. |
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| Keywords: | SRAP |
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