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| 小麦抗白粉病基因Pm6的RAPD标记和SCAR标记 | |
| 引用本文： | 许红星,刘红彦,李锁平,郅玉宝,杨共强,何文兰,宋玉立.小麦抗白粉病基因Pm6的RAPD标记和SCAR标记[J].河南农业科学,2004(4):25-28. |
| 作者姓名： | 许红星 刘红彦 李锁平 郅玉宝 杨共强 何文兰 宋玉立 |
| 作者单位： | 1. 河南大学生命科学学院,河南,开封,475001;河南省农业科学院植物保护研究所 2. 河南省农业科学院植物保护研究所 3. 河南大学生命科学学院,河南,开封,475001 4. 河南省农作物品种重点实验室 |
| 基金项目： | 河南省自然科学基金项目 (0 0 4 0 12 10 0 ) |
| 摘 要： | 用 1 8 1条 1 0碱基随机引物扩增感病亲本豫麦 1 3号和含Pm6基因的抗病亲本Tim galen ,有 37条引物在Timgalen中检测到多态性片断 ,经多次重复和F2 验证 ,引物S1 38仍能在Timgalen中扩增出稳定的多态性片断 ,对该多态性片断进行回收、克隆和测序 ,根据其序列合成 1对特异引物 ,成功地将RAPD标记转化成稳定的SCAR标记 ,并用此引物对豫麦 1 3号和Timgalen的杂交F2 单株检测 ,计算出该标记与抗病基因之间的遗传距离为 2 7.1cM。并对含不同抗白粉病基因的载体品种进行了SCAR分析。 |
| 关 键 词： | 小麦 抗白粉病基因 SCAR标记 RAPD标记 |
| 文章编号： | 1004-3268(2004)04-0025-04 |
| 修稿时间： | 2004年1月6日 |
RAPD and SCAR Markers Linked to Wheat Powdery Mildew Resistance Gene Pm6 |
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| XU Hong-xing ,LIU Hong-yan,LI Suo-ping,ZHI Yu-bao,YANG Gong-qiang,HE Wen-lan,SONG Yu-li.RAPD and SCAR Markers Linked to Wheat Powdery Mildew Resistance Gene Pm6[J].Journal of Henan Agricultural Sciences,2004(4):25-28. | |
| Authors: | XU Hong-xing LIU Hong-yan LI Suo-ping ZHI Yu-bao YANG Gong-qiang HE Wen-lan SONG Yu-li |
| Institution: | XU Hong-xing 1,2,LIU Hong-yan2,LI Suo-ping1,ZHI Yu-bao3,YANG Gong-qiang2,HE Wen-lan2,SONG Yu-li2 |
| Abstract: | decamer random primers were used to amplify between susceptible parent Yumai 13 and resistant parent Timgalen with Pm6 gene, and 37 primers of them could detect the polymorphic segment. Through several repeat and confirmation of small F 2 population derived from a cross between Timgalen and Yumai 13,one primer S138 produced reproducible segment only in the resistant parent Timgalen. The segment was recovered, cloned and sequenced. According to its sequence, a pair of special primer was synthesized and the RAPD marker was converted into a SCAR marker. Linkage analysis on the single plant of F 2 population, showed that SCAR 443 markers were linked in coupling to the resistance genes Pm6 at a distance of 27.1 cM. The marker SCAR 443 was use to screen a set of varieties carrying different Pm genes and its value was evaluated. |
| Keywords: | Wheat Powdery mildew resistantce gene SCAR markers RAPD markers |
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