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| 亚麻SSR-PCR优化体系的建立 | |
| 引用本文： | 王斌,张建平,党占海,赵丽娟,王利民,颉瑞霞.亚麻SSR-PCR优化体系的建立[J].甘肃农业大学学报,2010,45(4). |
| 作者姓名： | 王斌 张建平 党占海 赵丽娟 王利民 颉瑞霞 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070 2. 甘肃省农业科学院作物研究所,甘肃,兰州,730070 3. 甘肃省农业科学院生物技术研究所,甘肃,兰州,730070 |
| 基金项目： | 甘肃省农业生物技术研究与应用开发项目，甘肃省自然科学科学基金项目 |
| 摘 要： | 通过研究SSR-PCR反应体系的主要成分对扩增结果的影响,建立了亚麻SSR反应的优化体系,即总体积20μL,Mg2+1.9mmol.L-1,dNTPs 0.55mmol.L-1,Taq DNA聚合酶1.5U,25ng.μL-1引物75ng,DNA模板(50ng.μL-1)100ng.利用10个亚麻材料对优化后的SSR体系进行验证,1.5%琼脂糖检测结果显示,优化后的SSR体系稳定性,重复性好,扩增效果良好. |
| 关 键 词： | 亚麻 SSR 优化 |
Establishment and optimization of SSR-PCR reaction system in flax |
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| WANG Bin,ZHANG Jian-ping,DANG Zhan-hai,ZHAO Li-juan,WANG Li-min,XIE Rui-xia.Establishment and optimization of SSR-PCR reaction system in flax[J].Journal of Gansu Agricultural University,2010,45(4). | |
| Authors: | WANG Bin ZHANG Jian-ping DANG Zhan-hai ZHAO Li-juan WANG Li-min XIE Rui-xia |
| Abstract: | The factors which affected the SSR-PCR reaction results were studied in the experiment,the optimum SSR reaction system of flax was established,it was showed as follows:total volume of reaction was 20 μL,the optimum concentrations of Mg2+ and dNTPs were 1.9 mmol·L-1 and 0.55 mmol·L-1 respectively,moreover,1.5 U Taq DNA polymerase,50 ng·μL-1 template DNA 100ng,25 ng·μL-1 primer 75 ng.To evaluate the SSR system,10 different flax germplasm were used and tested by 1.5 % agarose gel,the PCR results showed that this SSR reaction system was steady and reproducible. |
| Keywords: | SSR |
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