| 窄冠白杨组培快繁与再生体系的建立 |
| |
| 引用本文： | 李际红,邢世岩,焦 振,刘国兴,张友朋.窄冠白杨组培快繁与再生体系的建立[J].中国农学通报,2008,24(7):60-64. |
| |
| 作者姓名： | 李际红 邢世岩 焦 振 刘国兴 张友朋 |
| |
| 作者单位： | 山东农业大学林学院,泰安,271018 |
| |
| 摘 要： | 以窄冠白杨为试材，对其组培快繁与再生体系的建立进行了初步的研究。研究表明，抗氧化剂聚乙烯吡咯烷酮（PVP）和抗坏血酸（Vc）能有效降低外植体的褐化；该品种外植体增殖的最适培养基为1/2MS+BA0.3mg/L+NAA0.05mg/L，平均分化率为87%，增殖系数达7.5倍，与其它处理组合均呈极显著差异。硝普钠（SNP）12mg/L极显著地促进叶片不定芽分化，其再生频率与再生芽个数分别达到96.5%与8.2。窄冠白杨适宜的生根培养基为1/2MS+IBA0.3mg/L。
|
| 关 键 词： | 共轭亚油酸 共轭亚油酸 多不饱和脂肪酸 免疫功能 |
| 收稿时间： | 2008-04-28 |
| 修稿时间： | 2008-05-27 |
Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidalis in vitro |
| |
| Li Jihong,Xing Shiyan,Jiao Zhen,Liu Guoxing,Zhang Youpeng.Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidalis in vitro[J].Chinese Agricultural Science Bulletin,2008,24(7):60-64. |
| |
| Authors: | Li Jihong Xing Shiyan Jiao Zhen Liu Guoxing Zhang Youpeng |
| |
| Institution: | College o f Forest Shandong Agriculture University, Tai’an 271018 |
| |
| Abstract: | In this report, tissue culture and regeneration system from leaves in vitro have been extensively investigated utilizing Populus Leucopyramidalis as experiment materials. The results showed that the anti-oxidation materials polyvinylpyrrolidone (PVP) and ascorbic acid (Vc) decreased browning efficiently; the best medium for proliferation was 1/2 MS+ BA0.3mg/L + NAA 0.05mg/L with differentiation percentage of 87% and proliferation multiples of 7.5 respectively, and there was a great significant difference compared with other treatments. The differentiation and regeneration of adventitious shoot from leaves increased significantly in the medium MS containing BA0.3mg/L and NAA0.05mg/L supplemented with 12mg/L sodium nitroprusside (SNP) in which regeneration frequency and average number of shoots were 100% and 12 respectively. The best rooting medium was 1/2 MS+ IBA0.3mg/L . |
| |
| Keywords: | populus leucopyramidaliszz tissue culturezz regeneration systemzz sodium nitroprussidezz |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
