| 影响非洲菊离体培养器官分化的因素 |
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| 引用本文： | 郑秀芳,王桔红,李名扬.影响非洲菊离体培养器官分化的因素[J].江苏林业科技,2002,29(1):29-31. |
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| 作者姓名： | 郑秀芳 王桔红 李名扬 |
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| 作者单位： | 1. 张掖师范专科学院生物系,甘肃,张掖,734000
2. 西南农业大学园艺系,重庆,400716 |
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| 摘 要： | 采用非洲菊的 4个不同切花品种 ,主要通过对花托外植体进行离体培养 ,从基因型、激素组合、基本培养基、蔗糖质量浓度等方面研究了非洲菊器官形成的规律。结果表明 :幼小的花托是非洲菊最适宜培养的外植体。虽然品种之间存在着差异 ,但在 2个月内均能从愈伤组织上直接产生不定芽。芽的产生以IAA 0 1mg/L及BA 10mg/L为最佳激素配比 ,而基本培养基诱导芽分化的效果是B5>1/ 2MS >MS ,1%蔗糖较之高质量分数蔗糖更有利于芽的分化。
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| 关 键 词： | 非洲菊 组织培养 芽分化 |
| 文章编号： | 1001-7380(2002)01-0029-03 |
| 修稿时间： | 2001年8月17日 |
Factors affecting organogenesis in Gerbera jamesonii Bolus cultures in vitro |
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| ZHENG Xiu fang ,WANG Ju hong ,LI Ming yang.Factors affecting organogenesis in Gerbera jamesonii Bolus cultures in vitro[J].Journal of Jiangsu Forestry Science & Technology,2002,29(1):29-31. |
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| Authors: | ZHENG Xiu fang WANG Ju hong LI Ming yang |
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| Institution: | ZHENG Xiu fang 1,WANG Ju hong 1,LI Ming yang 2 |
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| Abstract: | Factors affecting organogenesis in Gerbera jamesonii Bolus cultures in vitro were studied at levels of genotypes, hormones combination, basal media and sucrose concertration. The results in dicated that: Young capitulum was optimum explant for culture; for induction of the adventitious buds, extra hormones were necessary, the optimum hormones combination was 0 1 mg/L IAA and 10 mg/L BA; 1% sucrose was better for the bud differentiation than that of higher concentration; Among basal media, B 5 was the best, followed by 1/2MS, MS. |
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| Keywords: | Gerbera jamesonii Bolus Tissue culture Bud differentiation |
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