| 西葫芦离体雌核发育初期POD及其同工酶活性变化研究 |
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| 引用本文： | 王丽娜,王秀峰,谢冰,李晓京,王东红,彭云龙.西葫芦离体雌核发育初期POD及其同工酶活性变化研究[J].西北农业学报,2008,17(4):258-262. |
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| 作者姓名： | 王丽娜 王秀峰 谢冰 李晓京 王东红 彭云龙 |
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| 作者单位： | 山东农业大学园艺科学与工程学院,山东泰安,271018 |
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| 基金项目： | 山东省农业良种产业化工程项目 |
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| 摘 要： | 以一代杂种碧珠为供体诱导西葫芦离体雌核发育,研究了离体雌核发育初期胚珠中过氧化物酶(POD)及其同工酶活性的变化规律.结果显示,西葫芦离体雌核发育初期即未受精胚珠接种后13 d内,胚珠中POD及其同工酶活性变化明显,其中总的过氧化物酶(POD)、NADH过氧化物酶(NADHPOD)、生长素过氧化物酶(IAAO)、谷胱甘肽过氧化物酶(GSH-Px)的活性呈上升趋势;细胞色素过氧化物酶(COX)、抗坏血酸过氧化物酶(ASP)的活性呈先下降后略升高的趋势.
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| 关 键 词： | 西葫芦 离体雌核发育 过氧化物酶(POD) 同工酶 西葫芦 离体雌核发育 酶活性变化 研究 Stage Early Isoenzymes Peroxidase Activities pepo 抗坏血酸过氧化物酶 细胞色素 趋势 胱甘肽过氧化物酶 生长素 NADH 接种 未受精胚珠 显示 结果 |
| 收稿时间： | 2008/3/3 0:00:00 |
| 修稿时间： | 2008/3/31 0:00:00 |
Activities of Peroxidase and Its Isoenzymes in Early Stage of Summer Squash(Cucurbita pepo L.) Gynogenesis |
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| WANG Li-n,WANG Xiu-feng,XIE Bing,LI Xiao-jing,WANG Dong-hong and PENG Yun-long.Activities of Peroxidase and Its Isoenzymes in Early Stage of Summer Squash(Cucurbita pepo L.) Gynogenesis[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(4):258-262. |
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| Authors: | WANG Li-n WANG Xiu-feng XIE Bing LI Xiao-jing WANG Dong-hong and PENG Yun-long |
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| Institution: | College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China;College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China;College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China;College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China;College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China;College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China |
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| Abstract: | Activities of peroxidase and its isoenzymes during the early stage of gynogenesis were studied with summer squash (Cucurbita pepo L.) hybrid F1 "Bizhu".The activities of peroxidase and its isoenzymes changed obviously in unpollinated ovules cultured for 0-13 days.The results showed that the activity of total peroxidase (POD),NADH peroxidase(NADHPOD),indole-3-acetic acid peroxidase(IAAO),glutathione peroxidase(GSH-Px) were all increased gradually.While cytochrome peroxidase (COX) and ascorbate peroxidase (ASP) decreased first,then increased slightly. |
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| Keywords: | Summer squash (Cucubita pepo L ) Gynogenesis Peroxidase(POD) Isoenzymes |
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