| 姜黄素抑制轮状病毒感染猪肠上皮细胞的作用研究 |
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| 引用本文： | 卓儒浩,吴嘉敏,许梦慧,陶生祥,孟闯,钟翔.姜黄素抑制轮状病毒感染猪肠上皮细胞的作用研究[J].动物营养学报,2022,34(2):1285-1295. |
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| 作者姓名： | 卓儒浩 吴嘉敏 许梦慧 陶生祥 孟闯 钟翔 |
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| 作者单位： | 南京农业大学动物科技学院,南京 210095;江苏高校动物重要疫病与人兽共患病防控协同创新中心,扬州 225009;南京农业大学动物科技学院,南京 210095;江苏高校动物重要疫病与人兽共患病防控协同创新中心,扬州 225009 |
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| 基金项目： | 国家自然科学基金项目(31872391);江苏省人兽共患病学重点实验室资助项目(R2006)。 |
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| 摘 要： | 本研究旨在探讨姜黄素对猪轮状病毒(PRV)感染猪肠上皮细胞(IPEC?J2细胞)的抗病毒作用.以IPEC?J2细胞为试验对象,分别设置阴性对照组、感染PRV(感染复数=0.1)组和感染PRV后姜黄素(20μmol/L)处理组.在感染PRV后观察细胞病变,利用流式细胞术检测细胞内活性氧(ROS)含量,并通过实时荧光定量P...
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| 关 键 词： | 姜黄素 猪轮状病毒 猪肠上皮细胞 抗病毒 |
Inhibitory Effect of Curcumin against Rotavirus Infection in Intestinal Porcine Epithelial Cells |
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| ZHUO Ruhao,WU Jiamin,XU Menghui,TAO Shengxiang,MENG Chuang,ZHONG Xiang.Inhibitory Effect of Curcumin against Rotavirus Infection in Intestinal Porcine Epithelial Cells[J].Acta Zoonutrimenta Sinica,2022,34(2):1285-1295. |
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| Authors: | ZHUO Ruhao WU Jiamin XU Menghui TAO Shengxiang MENG Chuang ZHONG Xiang |
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| Institution: | (College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infection Diseases and Zoonoses,Yangzhou 225009,China) |
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| Abstract: | This study was explored the antiviral effect of curcumin on porcine rotavirus(PRV)-infected intestinal porcine epithelial cells(IPEC-J2 cells). IPEC-J2 cells were used as the experimental object and were assigned to the negative control group,the PRV infection(multiplicity of infection = 0.1)group,and the PRV infection with curcumin treatment(20μmol/L)group,respectively. After PRV infection,the cytopathic changes were observed,and the content of reactive oxygen species in IPEC-J2 cells was detected by flow cytometry,and the replication and proliferation of PRV in IPEC-J2 cells were detected by real-time fluorescence quantification(qRT-PCR)and virus titer assay. The results showed as follows:compared with a negative control group,1)PRV infection caused lesions of IPEC-J2 cells,significantly reduced cell viability(P<0.05),and significantly upregulated the content of intracellular ROS(P<0.01);2)curcumin could inhibit the replication and proliferation of PRV in IPEC-J2 cells(P<0.05);3)adding curcumin at the stage of PRV adsorption cells significantly inhibited the replication and proliferation of the virus(P<0.05);4)direct incubation of curcumin with PRV before infection could significantly reduce the titer of the virus after infection(P<0.05);5)PRV infection significantly increased the mRNA relative expression levels of melanoma differentiation related gene 5,interferon-induced protein 44-like protein antibody and interferon-β (P<0.05),and significantly reduced the mRNA relative expression levels of Toll-like receptor adapter molecule 1,mitochondrial antiviral signaling protein and myeloid differentiation factor 88 in IPEC-J2 cells(P<0.05),however,adding curcumin could significantly reduce or increase the expression of these immune gene affected by the virus(P<0.05). In summary,this study confirmed for the first time that curcumin can inhibit PRV infection and replication in intestinal porcine epithelial cells,providing important approach for prevention and treatment of piglet viral diarrhea.Chinese Journal of Animal Nutrition,2022,34(2):1285-1295] |
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| Keywords: | curcumin porcine rotavirus intestinal porcine epithelial cell antiviral effect |
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