| 猪睾丸组织与雄性生殖相关circRNA鉴定 |
| |
| 引用本文： | 刘锐,李江凌,陈晓晖,赵素君,王秋实,何志平,王宇萍.猪睾丸组织与雄性生殖相关circRNA鉴定[J].中国畜牧兽医,2021,48(10):3660-3669. |
| |
| 作者姓名： | 刘锐 李江凌 陈晓晖 赵素君 王秋实 何志平 王宇萍 |
| |
| 作者单位： | 1. 四川省畜牧科学研究院, 成都 610066;2. 动物遗传育种四川省重点实验室, 成都 610066;3. 四川省饲料工作总站, 成都 610041 |
| |
| 基金项目： | 院基本科研（SASA201909）；四川省科技计划（2021YFYZ0009、2021YFYZ0030） |
| |
| 摘 要： | 为研究环状RNA （circRNA）在杜洛克和大白猪睾丸中的表达差异，采用去除组织总RNA中核糖体RNA （rRNA）和线性RNA的方法构建特异性猪睾丸circRNA文库，并在Illumina PE150平台上进行测序，测序数据经过质控、比对、拼接后得到用于后续分析的数据。运用生物信息学软件find_circ和CIRI识别circRNA，并进行表达水平统计，从而得到猪睾丸组织circRNA表达谱，然后用DEseq2进行组间表达差异分析，对差异表达circRNA进行功能富集分析以筛选与雄性生殖相关的circRNA。结果发现，共识别到21 743个circRNAs，其中632个circRNAs在杜洛克和大白猪中的表达差异显著（|log2（FoldChange）|>1，P<0.05），在杜洛克猪上调表达的circRNAs有281个，下调表达的有351个。差异表达circRNA富集结果显示，有52个GO条目与繁殖相关，其中有9个与雄性生殖相关。经进一步筛选鉴定，有6个与雄性生殖相关的circRNAs （circ_0030058、circ_0009504、circ_00178101、circ_0019933、circ_0033379和circ_0017932），它们可能参与精原细胞分化、精子发生和精子细胞发育等生物学过程。综上所述，杜洛克和大白公猪睾丸中存在表达丰度不同的circRNA，这些circRNA可能参与精子生成，可以作为预测公猪生育能力的生物标记。
|
| 关 键 词： | 猪 睾丸 circRNA 精子生成 雄性生殖 |
| 收稿时间： | 2021-03-01 |
Identification of circRNA Related to Male Reproduction in Pig Testis |
| |
| LIU Rui,LI Jiangling,CHEN Xiaohui,ZHAO Sujun,WANG Qiushi,HE Zhiping,WANG Yuping.Identification of circRNA Related to Male Reproduction in Pig Testis[J].China Animal Husbandry & Veterinary Medicine,2021,48(10):3660-3669. |
| |
| Authors: | LIU Rui LI Jiangling CHEN Xiaohui ZHAO Sujun WANG Qiushi HE Zhiping WANG Yuping |
| |
| Institution: | 1. Sichuan Animal Sciences Academy, Chengdu 610066, China;2. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu 610066, China;3. Sichuan Animal Feed General Station, Chengdu 610041, China |
| |
| Abstract: | In order to study the differential expression of circRNA in Duroc and Yorkshire testis, a specific porcine testis circRNA library for sequencing was prepared using ribosomal RNA (rRNA) depletion and liner RNA digested method, and then sequenced on an Illumina PE150 platform. After quality control, comparison and splicing the sequencing data were obtained for subsequent analysis. The bioinformatics package find_circ and CIRI were used to identify circRNA and count the number of reads to obtain the cricRNA expression profile of porcine testis. Differential expression analysis of two groups was performed using the DESeq2 and then enrichment of those different expression circRNAs to screen which associate with male reproduction. The results showed that there was abundant expression of circRNA in testis. A total of 21 743 circRNAs were identified, and 632 circRNAs were significant differentially expressed in Duroc and Yorkshire pigs(|log2(FoldChange)|>1, P<0.05), 281 circRNAs up regulated and 351 circRNAs down regulated in Duroc pigs. The enrichment of difference expressed circRNA showed that there were 52 GO terms related to reproduction, and 9 of them related to male reproduction. 6 circRNAs related to male reproduction (circ_0030058, circ_0009504, circ_00178101, circ_0019933, circ_0033379 and circ_0017932) were screened out and they might take part in spermatogonia differentiation, spermatogenesis and sperm cell development. In conclusion, testis of Duroc and Yorkshire boars possess differentially expressed circRNA, which might be involved in spermatogenesis, and could also be used as biomarkers to predict boar fertility. |
| |
| Keywords: | pig testis circRNA spermatogenesis male reproduction |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
